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Hard Tissue paleometabolomics of early human sites yield metabolic and ecologic profiles - A: Metabolomics Data Set

ABSTRACT: The science of metabolic profiling exploits the chemical compound byproducts of biological functions called metabolites that, when analyzed, not only explain internal biological functions and physiological well-being, but also provide evidence of external influences specific to the habitat in which an organism lives. Here, we advance a new ultra fine scale bio molecular approach to understand the paleo environment of early human localities in eastern, central, and southern Africa using six fossil bones derived from 1.7-1.8 myr Bed I and 1.3 myr Bed II sites at Olduvai Gorge, Tanzania, one 2.4 myr sample from the Chiwondo Beds, Malawi, and one sample from the 3 myr site at Makapansgat, South Africa.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Kobus Ellipsiprymnus Defassa (ncbitaxon:91877) Kolpochoerus Majus Gerbilliscus Gentryi Dendromus Saccostomus Bubo Lacteus (ncbitaxon:126804) Xerus Inauris (ncbitaxon:234690) Gerbillus Sp. (ncbitaxon:10187) Elephas (ncbitaxon:9782) Xerus (ncbitaxon:226859) Bovidae (ncbitaxon:9895) Loxodonta Africana (ncbitaxon:9785) Mus Musculus (ncbitaxon:10090) Saccostomus (ncbitaxon:106346) Bubo Africanus (ncbitaxon:126801)

SUBMITTER: Dr. Thomas A. Neubert Dr. Timothy G.Bromage

PROVIDER: MSV000097146 | MassIVE | Tue Feb 18 16:12:00 GMT 2025

REPOSITORIES: MassIVE

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Project description:Esophageal squamous cell carcinoma (ESCC) is an aggressive cancer with one of the highest world incidences in the Eastern Cape region of South Africa. Several genome wide studies have been performed on ESCC cohorts from Asian countries, North America, Malawi and other parts of the world but none has been conducted on ESCC tumors from South Africa to date, where the molecular pathology and etiology of this disease remains unclear. We report here tumor associated copy number changes observed in 51 ESCC patients’ samples from the Eastern Cape province of South Africa. We extracted tumor DNA from 51 archived ESCC specimens and interrogated tumor associated DNA copy number changes using Affymetrix® 500K SNP array technology. The Genomic Identification of Significant Targets in Cancer (GISTIC) algorithm was applied to identify significant focal regions of gains and losses. Gains of the top recurrent cancer genes were validated by fluorescence in situ hybridization and their protein expression assessed by immunohistochemistry. Twenty-three significant focal gains were identified across samples. Gains involving the CCND1, MYC, EGFR and JAG1 loci recapitulated those described in studies on Asian and Malawian cohorts. The two most significant gains involved the chromosomal sub-bands 3q28, encompassing the TPRG1 gene and 11q13.3 including the CTTN, PPFIA1and SHANK2 genes. There was no significant homozygous loss and the most recurrent hemizygous deletion involved the B3GAT1 gene on chromosome11q25. Focal gains on 11q13.3 in 37% of cases (19/51), consistently involved CTTN and SHANK2 genes. Twelve of these cases (23,5%), had a broader region of gain that also included the CCND1, FGF19, FGF4 and FGF3 genes. SHANK2 and CTTN are co-amplified in several cancers, these proteins interact functionally together and are involved in cell motility. Immunohistochemistry confirmed both Shank2 (79%) and cortactin (69%) protein overexpression in samples with gains of these genes. In contrast, cyclin D1 (65%) was moderately expressed in samples with CCND1 DNA gain. This study reports copy number changes in a South African ESCC cohort and highlights similarities and differences with cohorts from Asia and Malawi. Our results strongly suggest a role for CTTN and SHANK2 in the pathogenesis of ESCC in South Africa.

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Hard Tissue paleometabolomics of early human sites yield metabolic and ecologic profiles - A: Metabolomics Data Set

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