Regulation of lipid metabolism gene expression in the C. elegans nervous system occurs via altering the repertoire of RNA binding proteins present on specific transcripts

ABSTRACT: Tissue-specific regulation of gene expression is essential for multicellular organisms, and RNA binding proteins play central roles in these molecular processes. To determine how the Caenorhabditis elegans RNA binding protein ADR-1 regulates tissue-specific gene expression, we profiled the binding targets of ADR-1 in neural cells and assessed the effects of ADR-1 RNA binding on neural gene expression. We identified a cohort of neural transcripts that facilitate lipid synthesis and are directly regulated by ADR-1 RNA binding. To identify cellular factors that influence ADR-1 binding, a forward genetic screen was performed, revealing that the serine/threonine protein kinase, glycogen synthase kinase-3 (GSK-3), inhibits ADR-1 binding to the cohort. Further investigation revealed that RNA binding protein VIG-1, promotes ADR-1 binding to the cohort in a GSK-3-dependent manner. Together, we reveal that interplay between kinases and RNA binding proteins regulates expression of lipid metabolism genes within neural cells, potentially impacting stress resistance and longevity.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Caenorhabditis Elegans (ncbitaxon:6239)

SUBMITTER: Heather Hundley   Amber L. Mosley  

PROVIDER: MSV000097188 | MassIVE | Fri Feb 21 11:14:00 GMT 2025

SECONDARY ACCESSION(S): PXD061064

REPOSITORIES: MassIVE