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Wu Selective Inhibition of Matrix Mechanosensing AP-MS

ABSTRACT: AP-MS of primary cardiac fibroblasts (Lonza, #CC-2904), 8 samples (n = 3 replicates per group): Undiff CF, MyoFb, TGFbi, Soft, Soft+TGFbi, SAR, SAR+TGFbi, IgG. The .d files acquired in data-independent mode were analyzed using Spectronaut v 19.1 (Biognosys AG, Schlieren, Switzerland) with the directDIA+ feature against the Uniprot Homo sapiens protein database. Proteolysis with Trypsin was assumed to be semi-specific, allowing for N-ragged cleavage with up to two missed cleavage sites, with a PSM false discovery rate (FDR) of 1%. Data filtering was based on the Q Value, and global normalization was utilized. Cysteine modified with iodoacetamide was set as a fixed modification. Variable modifications included oxidation of methionine and tryptophan, deamidation of asparagine and glutamine, and phosphorylation of serine, threonine, and tyrosine.

INSTRUMENT(S): timsTOF Ultra

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Arianne Caudal

PROVIDER: MSV000097212 | MassIVE | Tue Feb 25 11:28:00 GMT 2025

SECONDARY ACCESSION(S): PXD061188

REPOSITORIES: MassIVE

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