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Hepatocellular Metabolic Profile: Understanding Post-Thawing Metabolic Shift in Primary Hepatocytes in vitro

ABSTRACT: Positive and negative-mode metabolomics data from a time-course experiment for unexposed cells during a 24-hour time course, investigating the changes in metabolism of primary human hepatocytes in vitro post-thawing.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Emilio S. Rivera

PROVIDER: MSV000097484 | MassIVE | Tue Apr 01 14:07:00 BST 2025

REPOSITORIES: MassIVE

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Hepatocellular Metabolic Profile: Understanding Post-Thawing Metabolic Shift in Primary Hepatocytes In Vitro.

Palmisano Salvator S Breidenbach Joshua D JD Blackwell Brett R BR Harvey Tara T Luchini Kes A KA Thornhill M Grace MG LeBrun Erick S ES Mach Phillip P Glaros Trevor T Rivera Emilio S ES

Cells 20250529 11

Primary human hepatocytes (PHHs) are widely used as in vitro models for liver function and drug metabolism studies, yet their metabolic stability post-thawing remains an open question. To better characterize early metabolic changes, we conducted a time-course experiment using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to analyze metabolic shifts in PHHs cultured in suspension. Unexposed and exposed (acetaminophen-treated) samples were evaluated, and TITAN analysis was applied to d ...[more]

PMID: 40497979

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