Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Metabolomic analysis of stems of switchgrass lignin mutants under drought stress

ABSTRACT: This switchgrass project aims to identify metabolic consequences of lignin manipulation and impact on the responses to drought stress in switchgrass. Here we examine elongating internodes of six switchgrass lignin biosynthesis-altered transgenic and mutant and their isogenic wild-type negative segregant lines. Studies in the eudicot, Arabidopsis, have revealed metabolic and transcriptional differences in lignin biosynthesis mutants that appear to relate to feedback mechanisms for plants to compensate for lignin pathway disruption. Specifically, disruption of core lignin biosynthesis gene expression leads to increases in expression of cell wall-related pathways; whereas, disruption of downstream S- and G-lignin specific genes leads to down-regulation of lignin biosynthesis. This experiment allows us to test the generality of this observation in a bioenergy-relevant species. While we generally expect the same patterns, progress revealing differences in grass lignin biosynthesis pathways relative to eudicots suggests that we might also uncover differences. The work (proposal:https://doi.org/10.46936/10.25585/60000468) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Panicum Virgatum

SUBMITTER: Mariela Monteoliva

PROVIDER: MSV000097723 | MassIVE | Thu Apr 24 13:49:00 BST 2025

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Characterization of engineered sorghum with reduced S-adenosyl-L-methionine (AdoMet) levels

Project description:Lignin accumulates progressively in cell walls during plant development, therefore, we are interested in analyzing the transcriptome and metabolome of engineered sorghum lines at three developmental stages. Results from these analyses will help to understand the metabolic changes (if any) besides lignin synthesis in transgenics and anticipate plants' physiological responses during growth under natural environment in future field trials. This project will analyze the transcriptome and metabolome in stems of low-lignin sorghum transgenic lines compared to wild-type (WT) control. The work (proposal:https://doi.org/10.46936/10.25585/60008680) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

2024-05-14 | MSV000094762 | MassIVE

Elucidation of the Gene Regulatory Networks Governing Terpenoid-Mediated Plant-Environment Interactions to Enable Advanced Bioenergy Crops

Project description:The proposed project aims to elucidate the gene-regulatory networks (GRNs) underlying terpenoid metabolism in three major bioenergy crops, switchgrass (Panicum virgatum), maize (Zea mays), and sorghum (Sorghum bicolor), to aid crop development for enhanced stress resilience and biofuel production from biomass feedstock. Prior studies identified more than 50 terpene synthases (TPS) and cytochrome P450 monooxygenases (P450), as key enzymes in generating bioactive terpenoids, in several bioenergy plants including switchgrass and maize. This knowledge now enables a precise investigation of the GRNs that govern common and species-specific terpenoid pathways. We will integrate transcription factor (TF) functional studies, TF binding site mapping, transcript and metabolite profiling, and terpenoid pathway engineering to identify the terpenoid-metabolic TF networks mediating stress responses in switchgrass, maize and sorghum. The work (proposal:https://doi.org/10.46936/10.25585/60001378) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

2024-02-14 | MSV000094091 | MassIVE

Elucidating lignin catabolism in white rot fungi

Project description:We propose to elucidate new metabolic pathways in fungi - with a special focus on white rot fungi (WRF)- involved in the catabolism of lignin-derived aromatic compounds. Basidiomycete fungi, in particular WRF, are the most efficient organisms for the depolymerization and mineralization of lignin to CO2 and H2O in Nature and thus, WRF play a pivotal role in carbon cycling. Lignin depolymerization by WRF is mediated by the action of extracellular oxidative ligninolytic enzymes, such as laccases and peroxidases, alongside other secreted metabolites. This extracellular process has been studied for decades via analysis of lignin modifications, ligninolytic enzyme activity in fungal broths, enzyme isolation and characterization, and more recently through multi-omics analyses. Despite the massive research effort directed toward understanding how WRF depolymerize lignin, almost no attention has been dedicated to the elucidation of the intracellular metabolism of WRF in catabolizing lignin. In fact, whether or not WRF are able to catabolize lignin remains a matter of discussion and debate. The work (proposal:https://doi.org/10.46936/10.25585/60001176) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

2024-05-16 | MSV000094781 | MassIVE

Comparative and Population Genomics of Xylariaceae

Project description:We are examining the metabolites produced during co-culture of Xylariaceae fungi. The work (proposal:https://doi.org/10.46936/10.25585/60001144) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

2023-11-02 | MSV000093277 | MassIVE

Leveraging pan genomes to investigate diel transcriptomic and metabolomic responses to abiotic stress in Brassica. rapa and B. napus diversity panels.

Project description:Metabolomic analysis of the abiotic stress experiments on tissue from Brassicaceae genotypes. The work (proposal:https://doi.org/10.46936/10.25585/60001202) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

2023-11-02 | MSV000093268 | MassIVE

Exploring Genetics by Environment - 'omic analysis of engineered biomass crops in the lab and field

Project description:Clonal QSuB switchgrass plants (3 independent transformation events), and their corresponding wild type (var Alamo), grown in a growth chamber and the field, were harvested at two time points (five replicates) during the growing season across two years (2018, 2019), to compare the effects of environment on genetically identical engineered plants. To complement this, we used QsuB plus wild type for Arabidopsis grown in a walk in growth chamber. Three replicates of tissue will be harvested at a single time point. Plants will be well-watered or drought stressed. The work (proposal:https://doi.org/10.46936/10.25585/60000997) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

2023-11-02 | MSV000093270 | MassIVE

Homoserine lactone standards and heterologous expression of LuxI family synthases in E. coli

Project description:This dataset contains spectra of LuxI family synthases heterologously expressed and induced in E. coli as well as commercial homoserine lactones. The work (proposal:https://doi.org/10.46936/10.25585/60001376) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

2023-08-04 | MSV000092598 | MassIVE

Global metabolic profile comparison between 2 ecotypes upon inoculation with Azoarcus olearius

Project description:Arabidopsis thaliana associated with Plant Growth Promoting Bacteria (PGPB) Azoarcus olearius (DQS4) innoculated with WS (Wassilewskija) and Col0 (Columbia) ecotypes. The work (proposal:https://doi.org/10.46936/10.25585/60000448) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

2023-11-02 | MSV000093274 | MassIVE

Root metabolome characteristics of different root functional types in response to water and N limitations

Project description:One upland and one lowland switchgrass genotypes were grown in native soil under a rainout shelter and for three years under well-watered and water limited conditions. Further, two N treatments, + 67.25 kg N/ha and 0 fertilizer N application, were superimposed on the water availability treatments. Each of the 8 treatments (2 genotypes x 2 water availability x 2 N availability) was replicated three times with each experimental unit consisting of six plants in each of the treatments. Root and soil samples were collected at the end of the third year and roots were separated into transport and uptake roots to study how the metabolite profile in the two switchgrass ecotypes is influenced by water and N availability. This analysis will be coupled with a classification and identification of the root associated as well as rhizosphere microbiome. The work (proposal:https://doi.org/10.46936/10.25585/60001049) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

2025-04-24 | MSV000097718 | MassIVE

Exploring the role of drought-induced plant-associated microbes in promoting plant fitness in Sorghum bicolor and Oryza sativa

Project description:We investigate the role of root-associated Actinobacteria in promoting host fitness under drought stress in two plants important to the DOE mission of sustainable biofuels: Sorghum bicolor and Oryza sativa. The work (proposal:https://doi.org/10.46936/10.25585/60001083) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

2023-11-02 | MSV000093246 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data