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Untargeted Metabolomic Profiling of Streptomyces and Nocardia from Oligotrophic Ecosystems

ABSTRACT: We conducted an untargeted metabolomic analysis of Streptomyces and Nocardia strains isolated from two contrasting oligotrophic environments: Cuatro Cienegas and Calakmul. Metabolomic profiling was performed using liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-HR-MS/MS). Data acquisition was carried out in positive ionization mode under a Data-Dependent Acquisition (DDA) strategy. The analysis included samples cultivated under two distinct culture conditions: ISP2 and ISP5 media. Metabolites were extracted using a solvent mixture of acetonitrile, methanol, and ethyl acetate in a 1:1:1 ratio

INSTRUMENT(S): 6530A Q-TOF LC/MS

ORGANISM(S): Nocardia (ncbitaxon:1817) Streptomyces (ncbitaxon:1883)

SUBMITTER: Aldo Moreno Ulloa

PROVIDER: MSV000097984 | MassIVE | Fri May 23 17:43:00 BST 2025

REPOSITORIES: MassIVE

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