Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Eclipse
ORGANISM(S): Escherichia Coli (ncbitaxon:562) Homo Sapiens (ncbitaxon:9606) Desulfovibrio Marinus (ncbitaxon:370038)
SUBMITTER: Matthew P. DeLisa
Parastoo Azadi
PROVIDER: MSV000098017 | MassIVE | Wed May 28 09:54:00 BST 2025
SECONDARY ACCESSION(S): PXD064379
REPOSITORIES: MassIVE
Sotomayor Belen B Donahue Thomas C TC Mahajan Sai Pooja SP Taw May N MN Hulbert Sophia W SW Bidstrup Erik J EJ Owitipana D Natasha DN Pang Alexandra A Yang Xu X Ghosal Souvik S Alabi Christopher A CA Azadi Parastoo P Gray Jeffrey J JJ Jewett Michael C MC Wang Lai-Xi LX DeLisa Matthew P MP
Nature communications 20250704 1
Human immunoglobulin G (IgG) antibodies are a major class of biotherapeutics and undergo N-linked glycosylation in their Fc domain, which is critical for immune functions and therapeutic activity. Hence, technologies for producing authentically glycosylated IgGs are in high demand. Previous attempts to engineer Escherichia coli for this purpose have met limited success due in part to the lack of oligosaccharyltransferase (OST) enzymes that can install N-glycans at the conserved N297 site in the ...[more]