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Radulovic_2025_Response dynamics of discrete subiculum-retrosplenial cortex projections underlying trace fear conditioning

ABSTRACT: Associating temporally discontinuous events is one of the key characters of episodic memory. In trace fear conditioning (TFC), the CA1-subiculum (SUB) complex is required for tone-shock associations over a temporal trace, while the retrosplenial cortex (RSP) serves as a hub for temporal coding. How these regions interact during TFC remains unclear. Here, we show that molecularly distinct VGluT1 and VGluT2 SUB to RSP projections redundantly contribute to the associative component of TFC, whereas VGluT2 SUB to RSP projections selectively processes the temporal component. In these projections, trace memory formation was associated with increased and decreased bulk calcium activity at tone and trace onset, respectively, an activity pattern that was reestablished during memory recall. Such pattern was not observed in CA subfields, suggesting that associations and temporal gaps of TFC are integrated at the SUB before being presented to the RSP. Our findings establish a circuit mechanism for representing complex temporal information in episodic memory.

INSTRUMENT(S): Orbitrap Ascend

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: jeffrey Savas

PROVIDER: MSV000098056 | MassIVE | Fri May 30 14:14:00 BST 2025

SECONDARY ACCESSION(S): PXD064462

REPOSITORIES: MassIVE

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