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AP-MS of GFP-Lrrc56 from Xenopus laevis animal caps

ABSTRACT: Affinity Purification Mass Spectrometry from Xenopus laevis animal caps. Anti-GFP mmunoprecipitation was performed from embryo explants expressing either GFP (control) or GFP-Lrrc56. MS-MS data were collected using a DDA top speed stepped HCD method with a 60 minute gradient. The lookup database was a Uniprot X.laevis reference proteome collapsed into EggNOG vertebrate-level orthology groups. Details of methods in accompanying manuscript.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Xenopus Laevis (ncbitaxon:8355)

SUBMITTER: Edward Marcotte

PROVIDER: MSV000098174 | MassIVE | Thu Jun 12 12:02:00 BST 2025

REPOSITORIES: MassIVE

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