Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

HepG2 cells grown in antibiotics (PenStrep) versus non-antibiotic supplemented media

ABSTRACT: HepG2 cells grown for 9 passages in DMEM with 10% FBS and PenStrep antibiotics (A) or non-antibiotics (N) conditions. Cells were seeded at the same density from the same parent passage. Samples are named, e.g., P1_A5N1_3. P1 represents Plate 1, P2 represents Plate 2. The middle code represents conditions, e.g., grown in antibiotics-containing media for 5 passages then switched to non-antibiotic containing media for 1 passage. The final code represents the replicate (e.g., well #3 of a six well plate for this passage). KFB just represents a blank containing only beads during SP3 sample prep, no lysate. R represents reference (pooled) sample. Blanks represent just 0.1% formic acid in water. Hela represents ~500 ng HeLa

INSTRUMENT(S): Orbitrap Astral

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Jesse G. Meyer

PROVIDER: MSV000098243 | MassIVE | Tue Jun 17 15:51:00 BST 2025

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Secretome Analysis of Time- and Passage-Dependent of Wharton's Jelly Mesenchymal Stem Cells Using Quantitative Proteomic

Project description:This dataset contains proteins from the secretomes (Conditioned Media) of Wharton's Jelly Mesenchymal Stem Cells (WJMSC) harvested at different time points (24, 48, and 72 hours) and cell passages (Passage 3, Passage 5, and Passage 10). The samples used were taken from 2 donors. The research aims to understand how the composition of the secretome changes over time and with different cell passages. Advanced proteomic techniques are used to analyze the bioactive molecules secreted by WJMSCs, highlighting the impact of time and cell passage number on the therapeutic potential of these cells. This research seeks to enhance the clinical efficiency of WJMSCs for regenerative medicine applications by revealing the dynamic nature of their secretome.

| MSV000095902 | MassIVE

Global Transcription Profiling and Virulence Potential of Streptococcus pneumoniae after Serial Passage

Project description:Alterations in global transcriptional profiles of S. pneumoniae 6304 serotype 4 after 50 passages (50P) and 100 passages (100P) on laboratory media Keywords: Adaptation to unique growth environment Three conditions experiment: Control, single passage strain (1P), 2 biological replicates Experimental 50 passage strain (50P), 2 biological replicates Experimental 100 passage strain (100P), 2 biological replicates All strains independently grown and harvested at OD600 ~ 0.5. Four spot replicates per array.

2010-05-18 | E-GEOD-12607 | biostudies-arrayexpress

Single Cell Sequencing Reveals Gene Expression Signatures Associated with Bone Marrow Stromal Cell Subpopulations and Time in Culture [scRNA-seq]

Project description:Background Bone marrow stromal cells (BMSCs) are a heterogeneous population that participates in wound healing, immune modulation and tissue regeneration. Next generation sequencing was used to analyze transcripts from single BMSCs in order to better characterize BMSC subpopulations. Methods Cryopreserved passage 2 BMSCs from one healthy subject were cultured through passage 10. The transcriptomes of bulk BMSCs from designated passages were analyzed with microarrays and RNA sequencing (RNA-Seq). For some passages, single BMSCs were separated using microfluidics and their transcriptomes were analyzed by RNA-Seq. Results Transcriptome analysis microarray and RNA-Seq of unseparated BMSCs from passages 2, 4, 6, 8, 9 and 10 yielded similar results; both data sets grouped passages 4 and 6 and passages 9 and 10 together and genes differentially expressed among these early and late passage BMSCs were similar. 3D Diffusion map visualization of single BMSCs from passages 3, 4, 6, 8 and 9 clustered passage 3 and 9 into two distinct groups, but there was considerable overlap for passage 4, 6 and 8 cells. Markers for early passage, FGFR2, and late passage BMSCs, PLAT, were able to identify three subpopulations within passage 3 BMSCs; one that expressed high levels of FGFR2 and low levels of PLAT; one that expressed low levels of FGFR2 and high levels of PLAT and one that expressed intermediate levels of FGFR2 and low levels of PLAT. Conclusions Single BMSCs can be separated by microfluidics and their transcriptome analyzed by next generation sequencing. Single cell analysis of early passage BMSCs identified a subpopulation of cells expressing high levels of FGFR2 that might include skeletal stem cells.

2019-01-10 | GSE124858 | GEO

Single Cell Sequencing Reveals Gene Expression Signatures Associated with Bone Marrow Stromal Cell Subpopulations and Time in Culture [microarray]

2019-01-10 | GSE124860 | GEO

Single Cell Sequencing Reveals Gene Expression Signatures Associated with Bone Marrow Stromal Cell Subpopulations and Time in Culture [NGS_bulk cell RNA-seq]

2019-01-10 | GSE124859 | GEO

WU-BC 3, 4, and 5 PAM50 Subtypes with Passage and Gene Expression Comparison Between Xenografts and Primary Tumors

Project description:Whole genome expression studies were performed on Xenograft panels with multiple passages from 3 different patient tumors. We assessed the global gene expression concordance from passages to passage and from passage to primary tumor when available and performed PAM50 subtype classification. These results were examined within the context of 40 PAM50 prototype experiments.

2012-10-10 | GSE28860 | GEO

C. tropicalis cells under in vitro biofilm-forming conditions

Project description:Transcriptional profiling of a-type wor1 deleted cells and mixed a-type and alpha-type opaque cells under in vitro biofilm-forming conditions. Specifically, they were grown for two days at room temperature in a 12-well poly-styrene plate containing 1 ml of Lee's + Glucose liquid media. Samples were hybridized against a universal mixed reference sample of a-type cells in white and opaque states grown in Spider liquid media.

2013-11-22 | GSE52634 | GEO

Culture effects on rheumatoid arthrits synovial fibroblasts

Project description:Comparison between early passage to later passages of rheumatoid arthritis synovial fibroblast (RASF) populations using two different AtlasTM Human Arrays Gene expression changes during culture with increasing number of changes at higher passages depending on individual expression pattern of the respective cell populations

2010-04-20 | GSE21385 | GEO

Transcription profiling of human cells stored at ambient temperature in different storage media

Project description:This series represents the complete series of the human 293h media depleted storage on agarose / rehydration condition course analysis. Samples include Control, monolayer; Control, monolayer/full recovery, antibiotics; Spheroid, no storage; two week storage/0hr recovery; two week storage/full recovery; four week storage/0hr recovery; six week storage/0hr recovery.

2008-06-11 | E-GEOD-1364 | biostudies-arrayexpress

Long-Term Storage of Human Cells at Ambient Temperature

2005-08-01 | GSE1364 | GEO