CSF proteome mapping: Human Plasma LC-MS/MS, Part 6

ABSTRACT: 40 µL plasma from a pool of 20 neurologically healthy donors was separated into a bound and a depleted fraction using a MARS Hu-14 column. This experiment represents the bound fraction. Sample was in-solution trypsin digested and peptides were fractionated into 10 fractions by mixed-mode reversed phase anion exchange (MM(RP-AX) using a Promix MP column. Each fraction was analyzed separately by LC-MS/MS on an Orbitrap Velos Pro mass spectrometer. Resulting data was searched using SeachGui, summarized in PeptideShaker and exported to the CSF-PR.

INSTRUMENT(S): LTQ Orbitrap, MS:1000031

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Astrid Guldbrandsen  

PROVIDER: MSV000098346 | MassIVE | Fri Jun 27 00:42:00 BST 2025

SECONDARY ACCESSION(S): PXD000656

REPOSITORIES: MassIVE