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S. elongatus PCC 7942 Limited Proteolysis Structural Proteomics (JM-PB-DP3), LiP-MS-Optim

ABSTRACT: The purpose of this experiment was to investigate structural alterations in proteins involved in central carbon metabolism and photosynthetic electron transfer pathways in Synechococcus elongatus PCC 7942. Sample data was obtained from S. elongatus PCC 7942 cell lysates treated with different non-specific proteases (1:200 or 1:500 enzyme:substrate ratio) and prepared using limited proteolysis by mass spectrometry (LiP-MS) for global proteomic analysis. Tested proteases include: Proteinase K, Ficin, Thermolysin, Pronase, and/or Trypsin only. Peptides were identified using MaxQuant.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Synechococcus Elongatus Pcc 7942 (ncbitaxon:1140)

SUBMITTER: John T. Melchior Snigdha Sarkar

PROVIDER: MSV000098675 | MassIVE | Tue Jul 29 18:18:00 BST 2025

REPOSITORIES: MassIVE

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