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Zebrafish larvae proteome after exposure to tramadol and o-desmethyltramadol: raw data

ABSTRACT: The presence of pharmaceuticals in natural habitats is an increasing concern. In particular, the antidepressant tramadol (TRA) and its metabolite o-desmethyltramadol (OTRA). However, investigation of their impact on fish, particularly on their proteome, still needs attention. Zebrafish larvae were exposed to 0.1 or 100 ug/L of TRA or OTRA until reaching 168 hpf. The larvae proteome was then investigated through shotgun proteomics, employing the filter-aided sample preparation method (FASP) followed by high-throughput LC-MS/MS analysis. The work was partially supported by SIGNAL (COMPETE2030-FEDER-00877500, funded by Programa Inovação e Transição Digital (COMPETE 2030), Portugal 2030 and the Foundation for the Science and Technology (FCT), and by UIDB/04423/2020 and UIDP/04423/2020 programmes. Pedro Rodrigues was supported through FCT (2023.09236.CEECIND/CP2848/CT0003,https://doi.org/10.54499/2023.09236.CEECIND/CP2848/CT0003). Alexandre Campos and Mário Jorge Araújo also acknowledge the funding by for the Scientific Employment Stimulus Program (CEECIND/03767/2018 and CEECIND/CP2848/CT0005, https://doi.org/10.54499/2023.06491.CEECIND/CP2848/CT0005, respectively).

INSTRUMENT(S): Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer

ORGANISM(S): Danio Rerio (ncbitaxon:7955)

SUBMITTER: Antonio Paulo Carvalho Mario Jorge Araujo laura Guimaraes Pedro Rodrigues Alexandre Campos Luis Oliva Teles Maria V Turkina

PROVIDER: MSV000098727 | MassIVE | Tue Aug 05 09:22:00 BST 2025

REPOSITORIES: MassIVE

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