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Enabling spatial metabolomics and spatial proteomics from the same tissue section

ABSTRACT: We developed a workflow for comprehensive multi-omics profiling from a single tissue section (STS) using different mass spectrometry (MS)-modalities. We addressed key challenges associated with the need for serial sections and substrate incompatibilities between the modalities. We enhanced the functionality of an insulative substrate by employing metal-assisted approach, enabling its integration into dual applications for metabolite and proteomics imaging from STS using complementary MS-techniques. This allows for metabolite imaging using matrix-assisted laser desorption/ionization-MS imaging (MALDI-MSI) while preserving its compatibility for subsequent proteome profiling with laser capture microdissection (LCM)-based MS technology. Peptides were identified using DIA-NN.

INSTRUMENT(S): Orbitrap Astral

ORGANISM(S): Populus Trichocarpa (ncbitaxon:3694)

SUBMITTER: Dusan Velickovic

PROVIDER: MSV000098730 | MassIVE | Tue Aug 05 10:21:00 BST 2025

REPOSITORIES: MassIVE

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