Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Label free proteomics in M.smegmatis upon exposure to sub-inhibitory concentration of antibiotics_Streptomycin_25hrs

ABSTRACT: Differential expression analysis of total proteome upon streptomycin treatment in M.smegmatis at 25 hours

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mycolicibacterium Smegmatis (ncbitaxon:1772)

SUBMITTER: Raju Mukherjee

PROVIDER: MSV000098771 | MassIVE | Sat Aug 09 05:13:00 BST 2025

SECONDARY ACCESSION(S): PXD067148

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Label free proteomics in M.smegmatis upon exposure to sub-inhibitory concentration of Streptomycin_7.5hrs

Project description:Differential expression analysis of total proteome upon streptomycin treatment at 7.5hours in M.smegmatis

2025-07-04 | MSV000098432 | MassIVE

Label free proteomics in M.smegmatis upon exposure to sub-inhibitory concentration of antibiotics_Streptomycin_15hrs

Project description:Differential expression analysis of total proteome upon streptomycin treatment in M.smegmatis at 15 hours

2025-08-07 | MSV000098761 | MassIVE

Label free proteomics in M.smegmatis upon exposure to sub-inhibitory concentration of antibiotics_Norfloxacin_7p5hrs

Project description:Differential expression analysis of total proteome upon norfloxacin treatment at 7.5 hours in M.smegmatis

| MSV000099259 | MassIVE

Label free proteomics in M.smegmatis upon exposure to sub-inhibitory concentration of antibiotics_Norfloxacin_25hrs

Project description:Differential expression analysis of total proteome upon norfloxacin treatment in M.smegmatis at 25 hours

2025-08-24 | MSV000098929 | MassIVE

Label free proteomics in M.smegmatis upon exposure to sub-inhibitory concentration of antibiotics-Norfloxacin_15hours

Project description:Differential expression analysis of total proteome upon norfloxacin treatment in M.smegmatis at 15 hours

2025-08-21 | MSV000098909 | MassIVE

Transcriptome reveals the regulation on arginine metabolism in M.smegmatis.

Project description:Transcriptome reveals the regulation on arginine metabolism in M.smegmatis.

| PRJNA656135 | ENA

Label-free quantitative proteomic analysis of <i>Panax ginseng</i> leaves upon exposure to heat stress.

Project description:BackgroundGinseng is one of the well-known medicinal plants, exhibiting diverse medicinal effects. Its roots possess anticancer and antiaging properties and are being used in the medical systems of East Asian countries. It is grown in low-light and low-temperature conditions, and its growth is strongly inhibited at temperatures above 25°C. However, the molecular responses of ginseng to heat stress are currently poorly understood, especially at the protein level.MethodsWe used a shotgun proteomics approach to investigate the effect of heat stress on ginseng leaves. We monitored their photosynthetic efficiency to confirm physiological responses to a high-temperature stress.ResultsThe results showed a reduction in photosynthetic efficiency on heat treatment (35°C) starting at 48 h. Label-free quantitative proteome analysis led to the identification of 3,332 proteins, of which 847 were differentially modulated in response to heat stress. The MapMan analysis showed that the proteins with increased abundance were mainly associated with antioxidant and translation-regulating activities, whereas the proteins related to the receptor and structural-binding activities exhibited decreased abundance. Several other proteins including chaperones, G-proteins, calcium-signaling proteins, transcription factors, and transfer/carrier proteins were specifically downregulated.ConclusionThese results increase our understanding of heat stress responses in the leaves of ginseng at the protein level, for the first time providing a resource for the scientific community.

| S-EPMC6323179 | biostudies-literature

Development of Resistance in Escherichia coli ATCC25922 under Exposure of Sub-Inhibitory Concentration of Olaquindox.

Project description:Quinoxaline1,4-di-N-oxides (QdNOs) are a class of important antibacterial drugs of veterinary use, of which the drug resistance mechanism has not yet been clearly explained. This study investigated the molecular mechanism of development of resistance in Escherichia coli (E. coli) under the pressure of sub-inhibitory concentration (sub-MIC) of olaquindox (OLA), a representative QdNOs drug. In vitro challenge of E. coli with 1/100× MIC to 1/2× MIC of OLA showed that the bacteria needed a longer time to develop resistance and could only achieve low to moderate levels of resistance as well as form weak biofilms. The transcriptomic and genomic profiles of the resistant E. coli induced by sub-MIC of OLA demonstrated that genes involved in tricarboxylic acid cycle, oxidation-reduction process, biofilm formation, and efflux pumps were up-regulated, while genes involved in DNA repair and outer membrane porin were down-regulated. Mutation rates were significantly increased in the sub-MIC OLA-treated bacteria and the mutated genes were mainly involved in the oxidation-reduction process, DNA repair, and replication. The SNPs were found in degQ, ks71A, vgrG, bigA, cusA, and DR76-4702 genes, which were covered in both transcriptomic and genomic profiles. This study provides new insights into the resistance mechanism of QdNOs and increases the current data pertaining to the development of bacterial resistance under the stress of antibacterials at sub-MIC concentrations.

| S-EPMC7696260 | biostudies-literature

The Evolution of Fluoroquinolone Resistance in Salmonella under Exposure to Sub-Inhibitory Concentration of Enrofloxacin.

Project description:The evolution of resistance in Salmonella to fluoroquinolones (FQs) under a broad range of sub-inhibitory concentrations (sub-MICs) has not been systematically studied. This study investigated the mechanism of resistance development in Salmonella enterica serovar Enteritidis (S. Enteritidis) under sub-MICs of 1/128×MIC to 1/2×MIC of enrofloxacin (ENR), a widely used veterinary FQ. It was shown that the resistance rate and resistance level of S. Enteritidis varied with the increase in ENR concentration and duration of selection. qRT-PCR results demonstrated that the expression of outer membrane porin (OMP) genes, ompC, ompD and ompF, were down-regulated first to rapidly adapt and develop the resistance of 4×MIC, and as the resistance level increased (≥8×MIC), the up-regulated expression of efflux pump genes, acrB, emrB amd mdfA, along with mutations in quinolone resistance-determining region (QRDR) gradually played a decisive role. Cytohubba analysis based on transcriptomic profiles demonstrated that purB, purC, purD, purF, purH, purK, purL, purM, purN and purT were the hub genes for the FQs resistance. The 'de novo' IMP biosynthetic process, purine ribonucleoside monophosphate biosynthetic process and purine ribonucleotide biosynthetic process were the top three biological processes screened by MCODE. This study first described the dynamics of FQ resistance evolution in Salmonella under a long-term selection of sub-MICs of ENR in vitro. In addition, this work offers greater insight into the transcriptome changes of S. Enteritidis under the selection of ENR and provides a framework for FQs resistance of Salmonella for further studies.

| S-EPMC8619427 | biostudies-literature

The mechanism of tigecycline resistance in Acinetobacter baumannii under sub-minimal inhibitory concentration tigecycline

Project description:The mechanism of tigecycline resistance in Acinetobacter baumannii under sub-minimal inhibitory concentration tigecycline by comparative protemics

2024-01-26 | PXD048947 |

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data