Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Phosphoproteome-derived peptide libraries derived from human cell lines for phosphatase profiling

ABSTRACT: This dataset contains raw data corresponding to analysis of phosphoproteome-derived peptide libraries derived from untreated HEK293T cells and pervanadate-treated HEK293T, Jurkat, and K562 cells. It also contains a timecourse of lambda phosphatase treatment of a phosphoproteome-derived peptide library derived from pervanadate-treated HEK293T cells.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Amy Weeks

PROVIDER: MSV000098786 | MassIVE | Mon Aug 11 08:02:00 BST 2025

SECONDARY ACCESSION(S): PXD067199

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Supplementary Dataset 11c - SILAC data for HEK293T-Subtiligase-Y217D-TM Pervanadate treated vs untreated

Project description:This dataset contains SILAC data for HEK293T-subtiligase-Y217D-TM cells, pervanadate treated vs. untreated. In Q20180422-04, light cells were pervanadate treated and heavy cells were the control. In Q20180422-08 and Q20180422-10, heavy cells were pervanadate treated and light cells were the control.

2020-02-21 | MSV000084995 | MassIVE

Supplementary Dataset 11b - SILAC data for HEK293T-Subtiligase-Y217K-TM Pervanadate treated vs untreated

Project description:This dataset contains SILAC data for HEK293T-Subtiligase-Y217K-TM cells, pervanadate treated vs untreated. In Q20180417-03 and Q20180417-05, light cells were pervanadate treated and heavy cells were the control. In Q20180417-07 and Q20180417-09, heavy cells were pervanadate treated and light cells were the control.

2020-02-21 | MSV000084994 | MassIVE

Supplementary Dataset 11a - SILAC data for HEK293T-Subtiligase-TM Pervanadate treated vs untreated

Project description:This dataset contains SILAC data for HEK293T-subtiligase-TM cells, pervanadate treated vs. untreated. In Q20180412-02 and Q20180412-04, light cells were pervanadate treated and heavy cells were the control. In Q20180412-06 and Q20180412-08, heavy cells were pervandate treated and light cells were the control.

2020-02-20 | MSV000084992 | MassIVE

Phosphoproteome-derived peptide library profiling of human phosphatases PTP1B and PP2Ac

Project description:This dataset corresponds to timecourses of treatment of a pervandate-treated HEK293T phosphoproteome-derived peptide library with the human phosphatases PTP1B and PP2Ac.

2025-08-11 | MSV000098789 | MassIVE

Phosphoproteome-derived peptide library profiling of L. pneumophila phosphatases WipA and WipB

Project description:This dataset corresponds to timecourses of treatment of a pervandate-treated HEK293T phosphoproteome-derived peptide library with the Legionella pneumophila phosphatases WipA and WipB.

2025-08-11 | MSV000098792 | MassIVE

Phosphoproteome-derived peptide library profiling of bacterial phospholyases OspF, SpvC, and HopAI

Project description:This dataset corresponds to timecourses of treatment of a pervandate-treated HEK293T phosphoproteome-derived peptide library with the bacterial phospholyases OspF, SpvC, HopAI, or OspF(residues 25-239).

2025-08-11 | MSV000098793 | MassIVE

Phosphoproteome-derived peptide library profiling of OspF variants

Project description:This dataset corresponds to treatment of HEK293T phosphoproteome-derived peptide library with point variants of OspF to dissect the molecular basis of its specificity

2025-08-11 | MSV000098794 | MassIVE

RNA sequencing of HEK293T cells expressing peptide 9J10, a 14-3-3 interacting peptide

Project description:We investigated the transcriptome profile of HEK293T cells transfected with a plasmid encoding 9J10, a peptide isolated in a phenotypic screen from a library of peptides derived from bacterial and archaeal genomes. The peptide was identified in a screen for FOXO3a reactivation and has been shown to interact with 14-3-3 proteins and induce relocalisation of FOXO3a from the cytoplasm to the nucleus. A peptide with a single amino acid mutation does not interact with 14-3-3 and was included as a control. A constitutively active FOXO3a mutant was also included as a control.

2021-05-28 | E-MTAB-10161 | biostudies-arrayexpress

LL10000 hybridization

Project description:The screening of a previously reported fluorescein labelled 10,000 member PNA encoded peptide library allowed information on the interaction between the peptide-ligands and the cell surface receptors to be extracted, identified new peptide ligands for cell surface receptors, and gave crucial information about consensus sequences. A novel indirect amplification of the PNA signal by amplification of the PNA-complementary DNA library was developed to screen PNA-encoded peptide library against D54, HEK293T, and HEK293T-CCR6 cells. This work generates a new approach to biological discovery and an expansion of modern microarray techniques. In addition, the microarray approach facilitates screening for differences in surface-receptor ligands and/or receptor expression between various cell types including diseased and normal cells.

2011-11-08 | E-MEXP-3104 | biostudies-arrayexpress