Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Phosphoproteome-derived peptide library profiling of human phosphatases PTP1B and PP2Ac

ABSTRACT: This dataset corresponds to timecourses of treatment of a pervandate-treated HEK293T phosphoproteome-derived peptide library with the human phosphatases PTP1B and PP2Ac.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Amy Weeks

PROVIDER: MSV000098789 | MassIVE | Mon Aug 11 09:06:00 BST 2025

SECONDARY ACCESSION(S): PXD067202

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Phosphoproteome-derived peptide library profiling of L. pneumophila phosphatases WipA and WipB

Project description:This dataset corresponds to timecourses of treatment of a pervandate-treated HEK293T phosphoproteome-derived peptide library with the Legionella pneumophila phosphatases WipA and WipB.

2025-08-11 | MSV000098792 | MassIVE

Phosphoproteome-derived peptide library profiling of OspF variants

Project description:This dataset corresponds to treatment of HEK293T phosphoproteome-derived peptide library with point variants of OspF to dissect the molecular basis of its specificity

2025-08-11 | MSV000098794 | MassIVE

Phosphoproteome-derived peptide library profiling of bacterial phospholyases OspF, SpvC, and HopAI

Project description:This dataset corresponds to timecourses of treatment of a pervandate-treated HEK293T phosphoproteome-derived peptide library with the bacterial phospholyases OspF, SpvC, HopAI, or OspF(residues 25-239).

2025-08-11 | MSV000098793 | MassIVE

Phosphoproteome-derived peptide libraries derived from human cell lines for phosphatase profiling

Project description:This dataset contains raw data corresponding to analysis of phosphoproteome-derived peptide libraries derived from untreated HEK293T cells and pervanadate-treated HEK293T, Jurkat, and K562 cells. It also contains a timecourse of lambda phosphatase treatment of a phosphoproteome-derived peptide library derived from pervanadate-treated HEK293T cells.

2025-08-11 | MSV000098786 | MassIVE

DeepPhospho accelerates DIA phosphoproteome profiling through in silico library generation.

Project description:Phosphoproteomics integrating data-independent acquisition (DIA) enables deep phosphoproteome profiling with improved quantification reproducibility and accuracy compared to data-dependent acquisition (DDA)-based phosphoproteomics. DIA data mining heavily relies on a spectral library that in most cases is built on DDA analysis of the same sample. Construction of this project-specific DDA library impairs the analytical throughput, limits the proteome coverage, and increases the sample size for DIA phosphoproteomics. Herein we introduce a deep neural network, DeepPhospho, which conceptually differs from previous deep learning models to achieve accurate predictions of LC-MS/MS data for phosphopeptides. By leveraging in silico libraries generated by DeepPhospho, we establish a DIA workflow for phosphoproteome profiling which involves DIA data acquisition and data mining with DeepPhospho predicted libraries, thus circumventing the need of DDA library construction. Our DeepPhospho-empowered workflow substantially expands the phosphoproteome coverage while maintaining high quantification performance, which leads to the discovery of more signaling pathways and regulated kinases in an EGF signaling study than the DDA library-based approach. DeepPhospho is provided as a web server as well as an offline app to facilitate user access to model training, predictions and library generation.

| S-EPMC8602247 | biostudies-literature

Global phosphoproteome and transcriptome integration of Chlamydia trachomatis infection

Project description:Gene expression profiles of RNA extracted at 24 or 48h from End1 cells infected with Chlamydia trachomatis or uninfected controls. This experiment forms part of the analysis of phosphoproteome changes after C.trachomatis infection.

2019-01-16 | GSE104166 | GEO

Global phosphoproteome and transcriptome integration of Chlamydia trachomatis infection

Project description:Global phosphoproteome and transcriptome integration of Chlamydia trachomatis infection

| PRJNA411847 | ENA

Project description:InnateDB library HEK293T

| PRJNA1235432 | ENA

RNA sequencing of HEK293T cells expressing peptide 9J10, a 14-3-3 interacting peptide

Project description:We investigated the transcriptome profile of HEK293T cells transfected with a plasmid encoding 9J10, a peptide isolated in a phenotypic screen from a library of peptides derived from bacterial and archaeal genomes. The peptide was identified in a screen for FOXO3a reactivation and has been shown to interact with 14-3-3 proteins and induce relocalisation of FOXO3a from the cytoplasm to the nucleus. A peptide with a single amino acid mutation does not interact with 14-3-3 and was included as a control. A constitutively active FOXO3a mutant was also included as a control.

2021-05-28 | E-MTAB-10161 | biostudies-arrayexpress

Phosphoproteome

Project description:Phosphoproteome

2021-10-16 | OEX00011296 | NODE