Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

HDX-MS of UCH37 and Nb-ncS1 complex

ABSTRACT: Hydrogen deuterium exchange mass spectrometry data of UCH37 and Nb-ncS1 complex, uptake for ncS1 nanobody

INSTRUMENT(S): SYNAPT G2-Si

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Eric Strieter

PROVIDER: MSV000098855 | MassIVE | Mon Aug 18 01:58:00 BST 2025

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

HDX-MS of UCH37 and Nb-ncS1 complex

Project description:Hydrogen deuterium exchange mass spectrometry data of UCH37 and Nb-ncS1 complex, UCH37 uptake, raw files

2025-08-18 | MSV000098853 | MassIVE

HDX-MS of UCH37 and Nb-ncS1 complex

Project description:Hydrogen deuterium exchange mass spectrometry data of UCH37 and Nb-ncS1 complex, raw files

2024-11-17 | MSV000096438 | MassIVE

HDX-MS of UCH37 and Nb-cS1 complex

Project description:Hydrogen deuterium exchange mass spectrometry data of UCH37 and Nb-cS1 complex, uptake for UCH37, raw files

2025-08-18 | MSV000098852 | MassIVE

HDX-MS of UCH37 and Nb-cS1 complex

Project description:Hydrogen deuterium exchange mass spectrometry data of UCH37 and Nb-cS1 complex, raw files

2024-11-17 | MSV000096439 | MassIVE

RNA-Sequencing of Ncs1+/+ and Ncs1-/- mouse brain tissues

Project description:Analysis of genes and biological processes influenced by Neuronal calcium sensor 1 (NCS1) based on whole transcriptome analyiss of Ncs1+/+ (wildtype) and Ncs1-/- (knockout) mouse brain tissues, i.d. the frontal cortex and the hippocampus.

2021-09-29 | GSE171202 | GEO

IP-TMT-MS of Nb-ncS1 and Nb-cS1 transfected HEK293FT cells

Project description:IP-TMT-MS of Nb-ncS1 and Nb-cS1 transfected HEK293FT cells

2024-11-15 | MSV000096425 | MassIVE

Global TMT proteomics of Nb-ncS1 transfected HEK293FT cells

Project description:Global TMT proteomics of Nb-ncS1 transfected HEK293FT cells

2024-11-15 | MSV000096429 | MassIVE

RNA-Sequencing of Ncs1+/+ and Ncs1-/- mouse brain tissues

Project description:RNA-Sequencing of Ncs1+/+ and Ncs1-/- mouse brain tissues

| PRJNA718628 | ENA

HDX-MS analysis of a TTC7B specific nanobody to inhibit EFR3 mediated PI4KA recruitment

Project description:Phosphatidylinositol 4 kinase III (PI4KIII/PI4KA) is an essential lipid kinase that is critical for regulating plasma membrane identity. PI4KA is primarily recruited to the plasma membrane through targeted recruitment by the proteins EFR3A and EFR3B, with these binding to the PI4KA accessory proteins TTC7 (TTC7A and TTC7B) and FAM126 (FAM126A and FAM126B). Here we characterised how both EFR3 isoforms interact with all possible TTC7 and FAM126 combinations and developed a nanobody that specifically blocks EFR3 mediated PI4KA recruitment in TTC7B containing complexes. Using a yeast display approach, we generated a nanobody that is selective for TTC7B and blocks EFR3 binding. Cryo-electron microscopy and hydrogen deuterium exchange mass spectrometry reveal that the nanobody sterically blocks EFR3 recruitment and shows an extended interface with both PI4KA and TTC7B. Overall, this work provides insight into PI4KA regulation and is a useful tool for manipulating unique complexes of PI4KA that may be valuable for future therapeutic targeting of PI4KA.

2025-11-20 | PXD066213 | Pride

A Conformation specific Nanobody Targeting the NMN-activated State of SARM1

Project description:Upon axonal injury, Sterile alpha (SAM) and Toll/interleukin-1 receptor (TIR) motif containing 1 (SARM1) is activated by nicotinamide mononucleotide (NMN) to deplete NAD and consequently promote the process of axon degeneration (AxD). Currently, only the inactive form of SARM1 in its auto-inhibitory conformation has been resolved. The flexibility of the enzymatically active form of SARM1 has so far precluded its structural determination. To solve the problem, we generated a stabilizing nanobody, Nb-C6, that specifically recognized 30 only the NMN-activated form of SARM1. The conformation specificity was verified by immunoprecipitation and surface plasmon resonance. Fluorescently labeled Nb-C6 could immunostain only the activated SARM1 in cells stimulated with CZ-48, a permeant mimetic of NMN. Expression of Nb-C6 in live cells resulted in stabilization of the active form of the endogenous and exogenous SARM1, producing and elevating cellular levels of cyclic ADP-ribose, a calcium messenger. Cryo-EM of the NMN-activated SARM1 complexed by Nb-C6 showed an octameric structure resembling a “blooming lotus” with the ARM domains bending significantly inward and swinging out together with the TIR domains to form the “petals of the lotus”. Nb-C6 bound to the SAM domain of the activated SARM1 and stabilized its Armadillo repeat motif domain. Analyses using hydrogen-deuterium exchange mass spectrometry (HDX-MS), and cross-linking MS (XL-MS) indicate that the activated SARM1 is highly dynamic and flexible and the neighboring TIRs form dimers via the surface close to one BB loop. The Nanobody is thus a valuable tool for delineating the mechanism of activation of SARM1 in AxD and other cellular processes.

2022-11-29 | PXD033528 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data