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Li et al., Xylosyltransferase Bump-and-hole Engineering to Chemically Manipulate Proteoglycans in Mammalian Cells: CHO-pgs745 cells with 250 uM compound 1

ABSTRACT: CHO pgs-745 cells transfected with plasmids encoding for WT or bump-and-hole-engineered human XYLT1 or XYLT2 were fed with 6AzGlc-1-phosphate precursor (250 uM) or DMSO and secretome collected. Glycoproteins were clicked with acid-cleavable biotin-alkyne, enriched on neutravidin and on-bead digested with trypsin. Peptides were desalted and run on TIMS-ToF Pro.

INSTRUMENT(S): timsTOF Pro 2

ORGANISM(S): Cricetulus Griseus (ncbitaxon:10029)

SUBMITTER: Benjamin Schumann

PROVIDER: MSV000098977 | MassIVE | Fri Aug 29 14:13:00 BST 2025

SECONDARY ACCESSION(S): PXD067888

REPOSITORIES: MassIVE

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Li et al., Xylosyltransferase Bump-and-hole Engineering to Chemically Manipulate Proteoglycans in Mammalian Cells: CHO-pgs745 cells with 50 uM compound 1

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