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Large-scale growth for downstream purification of putative metallophores

ABSTRACT: This growth run led to two 1 L cultures, the first (LS1) was grown in MP (minimal media) with additions of 1% MeOH and 1% 464 (rich media). The second (LS2) was grown in 464 with 1% MeOH, then swapped into the same media as LS1 for 1 day prior to harvest as we hypothesized the extra growth may benefit cell numbers before swapping to minimal media to benefit downstream purification strategies.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Methylobacterium Aquaticum (ncbitaxon:270351)

SUBMITTER: Allegra Aron

PROVIDER: MSV000099114 | MassIVE |

REPOSITORIES: MassIVE

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