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Heat shock induces silent ribosomes and reorganizes mRNA turnover

ABSTRACT: mRNAs associate with single or multiple ribosomes these ribosomal assemblies monosomes and polysomes translate the mRNAs before degradation. The impact of heat stress on this mRNA turnover remains unclear. We show that in heat-shocked yeast cells, the proportion of monosomes increases without a corresponding rise in the number of associated mRNAs. Consequently, most monosomes are devoid of mRNAs and silent, lacking translational initiation factors and proteins facilitating posttranslational folding. Such silent monosomes also appear under other stress conditions, with proportions varying according to stress type, suggesting they represent a general feature of cellular adaptation. In parallel with the induction of silent ribosomes, elevated temperatures reduce the overall rate of mRNA ribosome association with few exceptions. Notably, heat-shock promotes the ribosomal association of transcripts encoding heatshock proteins, without extension of the halflives of these mRNAs. These mechanisms dynamically reorganize mRNA turnover to prioritize translation of heat-shock proteins over other proteins.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932)

SUBMITTER: Alexander Schmidt

PROVIDER: MSV000099165 | MassIVE |

SECONDARY ACCESSION(S): PXD068373

REPOSITORIES: MassIVE

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Heat shock induces silent ribosomes and reorganizes mRNA turnover

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