Project description:We sequenced mRNA from from four segments along a developing 9 day old third maize leaf of WT W22 accession and ZmDCT2 KO (in W22 background - dct2-1::Ac) plants, to identify changes in gene expression in dct2-1::Ac as a response to the loss of ZmDCT2 function. The segments were referenced relative to the position of the ligule of leaf 2 as this point represents the source-sink boundary in a wild-type leaf and the transition from shaded leaf tissue to fully light exposed. Segment -4 was isolated at the base of the leaf blade that is photosynthetically inactive, -1, defined as the sink to source transition zone (just below the point of leaf emergence from the whorl), segment +4, from a fully light exposed and photosynthetically active region in the mid-leaf (maturation zone), and +10, the tip of the leaf and the terminally differentiated tissue
Project description:Detect the global transcriptional changes occuring during spreading and maintenance of systemic post transcriptional silencing . Test the hypothesis that activation of systemic PTGS induces parallel antiviral defense pathways. Gene expression was analysed by MACE method (Massive Analysis of cDNA Ends) on total RNA extracted from leaf tissues of WT plants (WT), and GFP6.4 presenting no-silencing (NS sample), ongoing spreading of silencing (OS) and maintenance of silencing (SS). Plants were grown in parallel, and silencing state was monitored under UV. After 3 weeks of growth, total RNAs were extracted using the Trizol method from leaf tissues of 2-3 leaf stage plants. A total of 4 plants were sampled per variable (WT/NS/OS/SS). RNA from 4 plants were pooled and sequenced.
Project description:This SuperSeries is composed of the following subset Series: GSE14646: Gene expression in aerial parts of rice-Arabidopsis Os-LBD37 FOX plants GSE14647: Gene expression in leaf blades of the rice Os-LBD37 overexpressor line RK16331-13 Refer to individual Series
Project description:Lateral organ development is important for cucumber yield, while the molecular mechanism controlling leaf and floral organ development in cucumber remain elusive. In this report, a novel EMS-mutaginized mutant, round leaf (rl) was distinguished with remarkable round leaf shape, abnormal floral organ and inhibited tendril outgrowth in early development phase. Moreover, the ovule organogenesis disrupted completely in parthenocarpy fruit of rl. MutMap+ analysis revealed that RL encodes a protein kinase PINOID (CsPID, Csa1G537400). A non-synoymous SNP in the second exon of CsPID resulted in an amino-acid substitution from Arg in the wild type to Lys in the rl mutant. CsPID was down-regulated in rl mutant and preferentially expressed in young leaf, and flower buds. IAA quantification showed that rl plants exhibited a lower IAA content than wild type in ovary and blade edge. IAA immunolocalization results confirmed the IAA content alteration in rl plants. Transcriptome profile analysis further suggested IAA biosynthesis, polar transport and signal transduction genes participated in the leaf and floral development process by CsPID. Biochemical analyses showed that CsPID may regulate leaf shape by interacting with CsREV. In conclusion, this study revealed that the extensive genetic architecture of lateral organ organogenesis and development via CsPID regulating auxin polar transport action in cucumber.
Project description:Investigation of whole genome gene expression level changes in maize plants (standard maize line B73) in controlled conditions under continuous light. Tissues of the leaf elongation zone were sampled from plants well watered every 12 hours before and after lights on.
Project description:The 5th and 6th leaf blades of the rice Os-LBD37 overexpressor line RK16331-13 and the empty vector control line FOX3 were examined. LBD37 belongs to the plant- specific LOB- (Lateral Organ Boundary) domain family proteins first characterized in Arabidopsis. Results point towards an involvement of the rice LBD37 (OsLBD37) ortholog of Arabidopsis in nitrogen metabolism- and senescence- related processes. Hygromycin- resistant rice Os-LBD37 overexpressor and empty vector control plants were grown in hydroponic culture system. The sampling date of the leaf blades of RK16331-13 LBD37 overexpressor plants and the empty vector control was determined according to the emergence of the 8th leaf blade. Three independent hybridizations were performed for line RK16331-13 and the empty vector control line.
Project description:By implementing a leaf-removal treatment in the berry bunch zone, grapes were predominantly exposed to elevated light and their complete transcriptomes (as generated by RNASeq) were compared at four developmental stages.
Project description:UV-B radiation affects leaf growth in a wide range of species. In this work, we demonstrate that UV-B levels present in solar radiation inhibits maize leaf growth without causing any other visible stress symptoms, including accumulation of DNA damage. We conducted kinematic analyses of cell division and expansion to understand the impact of UV-B radiation on these cellular processes. Our results demonstrate that the decrease in leaf growth is a consequence of a reduction in cell production, and a shortened growth zone (GZ) in UV-B irradiated leaves. To determine the molecular pathways involved in UV-B inhibition of leaf growth, we performed RNA sequencing on isolated GZ tissues of control and UV-B exposed plants. Our results show a link between the observed leaf growth inhibition and the expression of specific cell cycle and developmental genes, including Growth Regulating Factors (GRFs) and transcripts for proteins participating in different hormone pathways.