Proteomics

Dataset Information

0

Characterisation of the Tilapia Lake Virus proteome

ABSTRACT: We investigated expression and localisation of all predicted proteins of Tilapia Lake Virus (TiLV) using in vitro translation, cell expression systems, and mass spectrometry, confirming major polypeptides from each segment and discovering an additional 11th protein, S9-F3, from an alternative reading frame. GFP-tagged constructs showed S2 and S10 localised mainly to the nucleus, while S1, S3, S5, S8 and S9-F3 were cytoplasmic, with S5 and S6 forming perinuclear foci. Bioinformatics and inhibitor assays revealed CRM1-dependent nuclear export of S9-F3. Evolutionary analyses indicated selective pressure on S9 and S9-F3 and identified an S9-F3 homologue in a TiLV-like guppy virus. These results reveal a novel protein and regulated nuclear export mechanism, offering new insights into TiLV biology and host interactions.

INSTRUMENT(S): timsTOF fleX

ORGANISM(S): Tilapia (ncbitaxon:8126)

SUBMITTER: Paul Digard  

PROVIDER: MSV000099204 | MassIVE |

SECONDARY ACCESSION(S): PXD068428

REPOSITORIES: MassIVE

Json Xml

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1
altmetric image

Publications

Characterisation of the tilapia lake virus proteome and identification of an 11th protein, S9-F3.

Pankaew Nunticha N   Kurian Dominic D   De Angelis Federico F   Del-Pozo Jorge J   Houston Ross D RD   Gratacap Remi L RL   Pinto Rute M RM   Digard Paul P  

Npj viruses 20260109 1

Tilapia Lake Virus (TiLV) is an emerging RNA virus threatening global tilapia aquaculture. While proteins encoded by segments 1-3 (polymerase) and 4 (nucleoprotein) are characterised, the functions of polypeptides from the remaining segments remain largely unknown. Here, we investigated the expression and subcellular localisation of all ten predicted TiLV proteins, confirming major polypeptides from each segment and identifying an additional 11th protein, S9-F3, translated from an alternative re  ...[more]

Similar Datasets

Genomics soil metagenome
| PRJNA244949 | ENA
Methylation profiling Methylome analysis of epiRIL344 BC1-S1 to BC1-S6 aerial part
2012-09-03 | GSE37285 | GEO
Transcriptomics The RNA exosome adaptor protein ZFC3H1 functionally competes with nuclear export activity to retain target transcripts
2018-05-14 | GSE108197 | GEO
Transcriptomics Methylome analysis of epiRIL344 BC1-S1 to BC1-S6 aerial part
2012-09-03 | E-GEOD-37285 | biostudies-arrayexpress
Transcriptomics NES_functional_survivin vs NES_mutated_survivin exposed or unexposed to temozolomide (TMZ)
2021-07-14 | GSE154337 | GEO
Transcriptomics Characterisation of the temporal hepatic transcriptome at subcellular level
2022-06-01 | GSE182856 | GEO
Transcriptomics Genome-wide analysis of gene expression and nuclear/cytoplasmic distribution by compound 1 treatment [293T-M]
2012-01-17 | E-GEOD-35112 | biostudies-arrayexpress
Transcriptomics Genome-wide analysis of gene expression and nuclear/cytoplasmic distribution by compound 1 treatment [293T_NS1-1]
2012-01-17 | E-GEOD-35113 | biostudies-arrayexpress
Transcriptomics Genome-wide analysis of gene expression and nuclear/cytoplasmic distribution by compound 1 treatment [293T_NS1-2]
2012-01-17 | E-GEOD-35114 | biostudies-arrayexpress
Transcriptomics Genome-wide analysis of gene expression by compound 1 treatment [HBEC_6_12hr]
2012-01-17 | E-GEOD-35116 | biostudies-arrayexpress