Proteomics

Dataset Information

0

Genomic analysis uncovers diverse Salmonella T6SS effectors and lipid-targeting antibacterial toxins


ABSTRACT: E. coli MG1655 containing the plasmids pBRA SP-Tox-Act1WT or SP-Tox-Act1C151A were cultured in LB and resuspended in AB supplemented with 0.2% L-arabinose to induce Tox-Act1 expression. Cell pellets were resuspended and the lipid-containing phase was extracted. Lipid extracts were analyzed using ultra-high-performance liquid chromatography (UHPLC Nexera, Shimadzu) coupled with an ESI-Q-TOF mass. MS and MS/MS data acquisition was performed using Analyst 1.7.1 software (Sciex). Mass spectrometry data was inspected using PeakView 2.0 software (Sciex), and lipid molecular species were manually identified with the help of an in-house manufactured Excel-based macro. Lipid species were quantified using MultiQuant software (Sciex), where the precursor ions areas were normalized by the internal standards for each class.

INSTRUMENT(S): Triple TOF 6600

ORGANISM(S): Escherichia Coli K-12

SUBMITTER: Robson Francisco de Souza   Gianlucca G Nicastro   Ethel Bayer-Santos  

PROVIDER: MSV000099245 | MassIVE |

REPOSITORIES: MassIVE

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

| S-EPMC5876133 | biostudies-literature
| S-EPMC10107278 | biostudies-literature
2021-01-26 | PXD021897 | Pride
2009-06-01 | GSE14005 | GEO
2010-06-10 | E-GEOD-5577 | biostudies-arrayexpress
| S-EPMC3652678 | biostudies-literature
2021-05-24 | PXD006295 | Pride
2023-06-21 | PXD043160 | JPOST Repository
2023-09-20 | GSE234601 | GEO
| S-EPMC9635880 | biostudies-literature