Genomic analysis uncovers diverse Salmonella T6SS effectors and lipid-targeting antibacterial toxins
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ABSTRACT: E. coli MG1655 containing the plasmids pBRA SP-Tox-Act1WT or SP-Tox-Act1C151A were cultured in LB and resuspended in AB supplemented with 0.2% L-arabinose to induce Tox-Act1 expression. Cell pellets were resuspended and the lipid-containing phase was extracted. Lipid extracts were analyzed using ultra-high-performance liquid chromatography (UHPLC Nexera, Shimadzu) coupled with an ESI-Q-TOF mass. MS and MS/MS data acquisition was performed using Analyst 1.7.1 software (Sciex). Mass spectrometry data was inspected using PeakView 2.0 software (Sciex), and lipid molecular species were manually identified with the help of an in-house manufactured Excel-based macro. Lipid species were quantified using MultiQuant software (Sciex), where the precursor ions areas were normalized by the internal standards for each class.
INSTRUMENT(S): Triple TOF 6600
ORGANISM(S): Escherichia Coli K-12
SUBMITTER:
Robson Francisco de Souza
Gianlucca G Nicastro
Ethel Bayer-Santos
PROVIDER: MSV000099245 | MassIVE |
REPOSITORIES: MassIVE
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