Dataset Information

Media supplementation experiment for metallophore production and transcriptional correlation

ABSTRACT: This dataset looks at putative metallophore production using 4 different media recipes. All were MP-based, but with different additives (media 464, casamino acids, and L-Trp).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Methylobacterium Aquaticum (ncbitaxon:270351)

SUBMITTER: Allegra Aron

PROVIDER: MSV000099321 | MassIVE |

REPOSITORIES: MassIVE

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Mandibular prognathism (MP) is the most common type of dento-maxillofacial deformity in East Asian populations. Genetic studies have revealed several MP-associated loci, suggesting that MP could be inherited as familial MP (fMP). However, functional verifications and in-depth mechanistic investigations of these loci are limited. For this study, we recruited 5 fMP families with 17 fMP members and 7 normal members. We first compared the clinical features of the 17 fMP members with 31 non-familial MP patients, finding a stronger mandibular overgrowth phenotype in the fMP subjects. Next, we performed whole-exome sequencing (WES) analysis with members of the 5 fMP families and singled out a potential fMP-associated pathogenic variant in the CASR gene—namely, rs117375173—the mutation introduces an amino acid substitution (A601G) in exon 7 and confers gain-of-function in Calcium-Sensing Receptor (CaSR). The rs11735173 variant changes the CaSR protein structure toward a semi-active state, similar to CaSR activated by L-tryptophan (L-Trp). To verify the regulating roles of CASR in mandibular bone growth, we further generated different mouse models with abnormal CaSR function. L-Trp administration effectively activate CaSR/GNAQ expression in vivo and in vitro. The MC3T3-E1 cell line transfected with CaSR with rs117375173 showed increased osteogenic differentiation and collagen synthesis at transcriptional level. Local injection of L-Trp in the mandible of growing mice significantly increased the mandibular length and BMD, due to activated osteogenic activity and suppressed bone resorption. At the same time, loss-of-function of CaSR in osteogenic progenitors caused mandibular growth retardation in Gli1-CreER; Casrfl/fl; tdTomatofl/+ mice. In conclusion, our study reveals that abnormal functioning of CaSR affects mandibular bone development and may contribute to the pathogenesis of fMP, providing a theoretical and experimental basis for the early diagnosis of and therapeutic strategies for fMP in clinical practice.

Dataset Information

Media supplementation experiment for metallophore production and transcriptional correlation

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets