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The Organ_resolved Metabolome Atlas of the Aging Mice_Lipidomics_POS and NEG

ABSTRACT: We profiled lipids across multiple organs and biofluids from C57BL/6NCrl (B6NCrl-MVP) wild-type mice at two ages (16 and 92 weeks), including both sexes. Samples spanned the nervous (hippocampus, cerebrospinal fluid), connective (brown, subcutaneous, and visceral/abdominal fat), digestive (stomach, duodenum, jejunum, ileum, cecum, colon, liver, gallbladder), immune (spleen, thymus), reproductive (testes, uterus), excretory (kidney, bladder), endocrine (pancreas), cardiovascular (heart), muscular (quadriceps), and respiratory (lung) systems, plus biofluids (plasma, urine) and digestive products (feces). Lipidomics was performed by LC-MS/MS on a Thermo Scientific Orbitrap Exploris 240 coupled to a Vanquish UHPLC in both positive and negative ion modes. Analytes were separated on a Waters ACQUITY Premier BEH C18 column (50 x 2.1 mm, 1.7 um) prior to high-resolution MS acquisition.

INSTRUMENT(S): Orbitrap Exploris 240

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Oliver Fiehn

PROVIDER: MSV000099388 | MassIVE | Sun Oct 05 12:47:00 BST 2025

REPOSITORIES: MassIVE

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