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Seed embryo and seed coat/endosperm specialized metabolome of Arabidopsis thaliana WT and aop3 mutant seeds developed under high temperature

ABSTRACT: Arabidopsis thaliana (thale cress) wild-type (WT) and transgenic lines were in the Columbia-0 (Col-0) background. The T-DNA insertion mutants of AOP3 (SALK_022752C), the WT were sowed directly in fertilized soil. Plants were grown in optimal conditions (control) in a growth chamber with long day photoperiod (16h light/21 celsius degrees - 8h dark/19 celsius degrees). Watering was made with water. At the onset of flowering (occurring between 5 and 6 weeks after seed sowing), high temperature (HT) was applied to half of the plants of the corresponding experiment by transferring them to another growth chamber (SANYO, Thermo Fisher Scientific) displaying high temperature conditions (16h of light/27 celsius degrees - 8h of dark/24 celsius degrees). The other half of the plants, constituting control plants, remained in the initial growth chamber with control temperature conditions. Dry seeds were harvested. Untargeted metabolomics (LC-MS/MS) were performed with those samples

INSTRUMENT(S): impact II, Ultimate 3000 (Thermo Fischer Scientific - HPLC)

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Massimiliano CORSO

PROVIDER: MSV000099458 | MassIVE | Mon Oct 13 02:01:00 BST 2025

REPOSITORIES: MassIVE

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