MiProChip A microfluidic device for high-throughput single-cell proteomics via multiplexed isobaric labeling

ABSTRACT: which is character 311: The MiProChip project presents a microfluidic platform for high-throughput single-cell proteomics, integrating on-chip lysis, digestion, and TMT-based labeling to achieve efficient and low-contamination proteome processing. This dataset includes (1) manually processed single-cell mimic samples (cell dilution x3 and peptide dilution x3) and MiProChip-processed single-cell samples from human NSCLC cell lines (PC9 and H1975), and (2) mouse MC38 cells treated with galectin-8, TGF-beta, or the combined treatment, comprising 40 single-cell datasets across five raw files. All data above were acquired on an Orbitrap Eclipse in DDA mode and analyzed using Proteome Discoverer with the CHIMERYS search engine. For the optimization experiment: 1. the acetonitrile percentage of TMT0 labeling results, Surface passivation testing (BSA vs DDM), and protease inhibitor removal testing were acquired on an Orbitrap Fusion Lumos in DDA mode and analyzed using Proteome Discoverer with the Sequest HT search engine.

INSTRUMENT(S): Orbitrap Eclipse, Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090)

SUBMITTER: Hsiung-Lin Tu   Yu-Ju Chen  

PROVIDER: MSV000099763 | MassIVE | Wed Nov 05 11:32:00 GMT 2025

SECONDARY ACCESSION(S): PXD070372

REPOSITORIES: MassIVE