Crosslinking MS analysis of GST-M18BP1(312-490) and CENP-HIKM using DSBU

ABSTRACT: Crosslinking mass spectrometry was performed to identify intermolecular DSBU crosslinks between GST-M18BP1(312-490) and the CENP-HIKM complex. The study aimed to map the binding interface of CENP-HIKM and M18BP1, comparing wild-type and a 355-359A M18BP1 mutant that no longer binds CENP-HIKM.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Petra Janning  

PROVIDER: MSV000099772 | MassIVE | Thu Nov 06 04:22:00 GMT 2025

SECONDARY ACCESSION(S): PXD070401

REPOSITORIES: MassIVE