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The Borreliella burgdorferi BosR-associated small non-coding RNA BasA regulates virulence

ABSTRACT: A01- 5A4 NP1 uninduced (32C, pH 7.6, 1% CO2) replicate 1 (TMTpro label 126) A02- 5A4 NP1 delta BasA uninduced (32C, pH 7.6, 1% CO2) replicate 1 (TMTpro label 127N) A03- 5A4 NP1 delta BasA complement uninduced (32C, pH 7.6, 1% CO2) replicate 1 (TMTpro label 127C) A04- 5A4 NP1 induced (37C, pH 6.8, 5% CO2) replicate 1 (TMTpro label 128N) A05- 5A4 NP1 delta BasA induced (37C, pH 6.8, 5% CO2) replicate 1 (TMTpro label 128C) A06- 5A4 NP1 delta BasA complement induced (37C, pH 6.8, 5% CO2) replicate 1 (TMTpro label 129N) B01- 5A4 NP1 uninduced (32C, pH 7.6, 1% CO2) replicate 2 (TMTpro label 129C) B02- 5A4 NP1 delta BasA uninduced (32C, pH 7.6, 1% CO2) replicate 2 (TMTpro label 130N) B03- 5A4 NP1 delta BasA complement uninduced (32C, pH 7.6, 1% CO2) replicate 2 (TMTpro label 130C) B04- 5A4 NP1 induced (37C, pH 6.8, 5% CO2) replicate 2 (TMTpro label 131N) B05- 5A4 NP1 delta BasA induced (37C, pH 6.8, 5% CO2) replicate 2 (TMTpro label 131C) B06- 5A4 NP1 delta BasA complement induced (37C, pH 6.8, 5% CO2) replicate 2 (TMTpro label 132N) C01- 5A4 NP1 uninduced (32C, pH 7.6, 1% CO2) replicate 3 (TMTpro label 132C) C02- 5A4 NP1 delta BasA uninduced (32C, pH 7.6, 1% CO2) replicate 3 (TMTpro label 133N) C03- 5A4 NP1 delta BasA complement uninduced (32C, pH 7.6, 1% CO2) replicate 3 (TMTpro label 133C) C04- 5A4 NP1 induced (37C, pH 6.8, 5% CO2) replicate 3 (TMTpro label 134N) C05- 5A4 NP1 delta BasA induced (37C, pH 6.8, 5% CO2) replicate 3 (TMTpro label 134C) C06- 5A4 NP1 delta BasA complement induced (37C, pH 6.8, 5% CO2) replicate 3 (TMTpro label 135N)

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Borrelia Burgdorferi B31 (ncbitaxon:224326)

SUBMITTER: Jon T. Skare

PROVIDER: MSV000099829 | MassIVE | Mon Nov 10 13:46:00 GMT 2025

REPOSITORIES: MassIVE

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Project description:In this research we present a transcriptomics analysis of the physiological response of a marine calcifier, Strongylocentrotus purpuratus, to ocean acidification, a decline in ocean pH that results from the absorption of anthropogenic carbon dioxide (CO2). Larvae were raised from fertilization to prism stage in seawater with elevated CO2 conditions based upon IPCC emissions scenario B1 (540ppm CO2) and A1FI (1020ppm CO2). Adult S. purpuratus were collected using SCUBA from Goleta Pier (Goleta,CA, USA) and maintained in flowing seawater tables at 15-16°C. Spawning was induced by coelomic injection of 0.5M KCl following standard methods (Strathmann 1987). Eggs were collected from 3 females and separately fertilized by sperm from a single male (i.e. all larvae were full or half-siblings). Each of the three replicate cultures were divided in three (nine cultures total) and held in 10L flow-through containers filled with seawater aerated through a side arm with commercially manufactured air containing three different pCO2: 380ppm CO2 (present day atmospheric CO2 level, pH 8.01 ± 0.01), 540ppm CO2, an optimistic atmospheric CO2 concentration predicted by the Intergovernmental Panel on Climate Change for 2100 (B1 scenario, pH 7.96 ± 0.01) and 1020ppm CO2 (A1FI scenario, pH 7.88 ± 0.01). Larvae were cultured at 15°C until prism stage (40h post-fertilization). At the 40 hour time point, a sample of 60,000 larvae was removed from each of the 9 cultures in the 380, 540 and 1020 ppm treatments and stored in 1 mL Trizol at -80 ºC for later RNA analysis. The larval cDNA sample from each replicate 540ppm CO2 culture was competitively hybridized against the 380ppm CO2 control culture from the same replicate female. Similarly, the larval cDNA sample from each replicate 1020ppm CO2 culture was competitively hybridized against the 380ppm CO2 control culture from the same replicate female. Using dye swaps of technical replicates, treatment effects could be estimated independently of dye effects.

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The Borreliella burgdorferi BosR-associated small non-coding RNA BasA regulates virulence

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