Systematic and Quantitative Investigation of Newly Synthesized Proteins Reveals Distinct Ion Homeostasis and Mitochondrial Changes between Cuproptosis and Ferroptosis in Human Cells

ABSTRACT: To better understand ferroptosis and cuproptosis, here we systematically and quantitatively analyzed newly synthesized proteins (NSPs), which reflect rapid changes in gene expression during cell death without the interference from the existing proteins. Integrating metabolic labeling with multiplexed proteomics, the results revealed that both types of cell death shared some common features, such as mitochondrial disorder and gene expression suppression. Furthermore, different changes between them were also found.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Ronghu Wu  

PROVIDER: MSV000099889 | MassIVE | Thu Nov 13 06:50:00 GMT 2025

SECONDARY ACCESSION(S): PXD070687

REPOSITORIES: MassIVE