Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomic analysis of human coronavirus 229E infected immortalized human lung fibroblasts (MRC5) at 8, 16, and 24 hours post infection (whole cell lysate)

ABSTRACT: PISA assays performed on mock-infected and human coronavirus 229E-infected immortalized human lung fibroblasts (MRC5) cells (BTOL0001590). Samples were harvested at 8, 16, and 24 hours post infection (HCoV-299E). Samples are derived from human whole cell lysates (BTO:0004304). Mock-infected and human coronavirus 229E-infected immortalized human lung fibroblasts (MRC5) cells were collected at 8, 16, and 24 hours post infection (HCoV-299E). Cells were lysed by freeze thaw cycles and each replicate was split in half and treated for 1 minute with proteinase K and then trypsin and the other half just treated with trypsin prior to processing for proteomic analysis. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Human Coronavirus 229e (ncbitaxon:11137) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: John T. Melchior Amy Sims

PROVIDER: MSV000099899 | MassIVE | Thu Nov 13 17:51:00 GMT 2025

SECONDARY ACCESSION(S): PXD070705

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Human A549 and MRC5 Cell Response to HCoV-229E Infection

Project description:The purpose of this experiment was to evaluate the human host cellular response to wild-type Human coronavirus strain 229E (HCoV-229E) infection. Sample data was obtained for mock and infected (MOI 5) immortalized human lung epithelial cells (A549) and immortalized human lung fibroblasts cells (MRC5) processed for RNA sequencing (RNA-Seq) expression analysis.

2025-10-01 | GSE279271 | GEO

Proteomic analysis (limited proteolysis and abundance based) of human coronavirus 229E infected immortalized human lung fibroblasts (MRC5) at 8, 16, and 24 hours post infection (whole cell lysate)

Project description:LiP assays performed on mock-infected and human coronavirus 229E-infected immortalized human lung fibroblasts (MRC5) cells (BTOL0001590). Samples were harvested at 8, 16, and 24 hours post infection (HCoV-299E). Samples are derived from human whole cell lysates (BTO:0004304). Mock-infected and human coronavirus 229E-infected immortalized human lung fibroblasts (MRC5) cells were collected at 8, 16, and 24 hours post infection (HCoV-299E). Cells were lysed by freeze thaw cycles and each replicate was split in half and treated for 1 minute with proteinase K and then trypsin and the other half just treated with trypsin prior to processing for proteomic analysis. The database searching was performed using FragPipe equipped with MSFragger, following the label-free quantification and match between runs (LFQ-MBR) workflow. The database search results were further processed using in-house codes which filtered and normalized the data, and added statistical parameters.

2025-11-13 | MSV000099901 | MassIVE

Human coronavirus-229E infection alters the structure of host RNA processing complexes

Project description:The purpose of this experiment was to evaluate the human host cellular response to wild-type human coronavirus strain 229E (HCoV-229E)(ncbitaxon:11137) infection. Sample data was obtained for mock and infected (MOI 3) immortalized human lung epithelial cells (A549), immortalized human lung fibroblasts cells (MRC5), and primary human airway epithelial cells from lung tissue, and processed for proteome expression analysis using Limited Proteolysis (LiP) methods for Tandem Mass Tag 16-Plex (TMT16) and global proteomic measurements. Sample data was acquired using a Q-Exactive HF-X mass spectrometer and data was processed and compiled using MaxQuant software (v1.6.17.0).

2024-10-31 | MSV000096268 | MassIVE

Human coronavirus-229E infection alters the structure of host RNA processing complexes

2024-11-05 | MSV000096303 | MassIVE

Human Primary Airway Epithelium Response to HCoV-229E Infection

Project description:The purpose of this experiment was to evaluate the human host cellular response to wild-type Human coronavirus strain 229E (HCoV-229E) infection. Sample data was obtained for mock and infected (MOI 3) primary human airway epithelial cells grown in air-liquid interface conditions, processed for RNA sequencing (RNA-Seq) expression analysis.

2025-10-01 | GSE279463 | GEO

Human Primary Airway Epithelium Response to HCoV-229E Infection in the Presence and Absence of Human Macrophages

Project description:The purpose of this experiment was to evaluate the human host cellular response to wild-type Human coronavirus strain 229E (HCoV-229E) infection in the presence and absence of macrophages. Sample data was obtained for mock and infected (MOI 3) primary human airway epithelial cells with and without macrophages and grown in air-liquid interface conditions, processed for RNA sequencing (RNA-Seq) expression analysis.

2025-10-01 | GSE280019 | GEO

Transcriptomic profiling of human corona virus (HCoV)-229E -infected human cells

Project description:Human coronaviruses (HCoVs) cause mild to severe respiratory infection. Most of the common cold illnesses are caused by one of four HCoVs, namely HCoV-229E, HCoV-NL63, HCoV-HKU1 and HCoV-OC43. Several studies have applied global transcriptomic methods to understand host responses to HCoV infection, with most studies focusing on the pandemic severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome CoV (MERS-CoV) and the newly emerging SARS-CoV-2. In this study, Next Generation Sequencing was used to gain new insights into cellular transcriptomic changes elicited by alphacoronavirus HCoV-229E. HCoV-229E-infected MRC5 cells showed marked downregulation of superpathway of cholesterol biosynthesis and eIF2 signaling pathways. Moreover, upregulation of cyclins, cell cycle control of chromosomal replication, and role of BRCA1 in DNA damage response, alongside downregulation of the cell cycle G1/S checkpoint, suggest that HCoV-229E favors S phase for viral infection. Intriguingly, more than 80% of key factors of cell innate immunity, interferon-stimulated genes (ISGs) and other transcripts of early antiviral response genes were downregulated early in HCoV-229E infection. This study will enhance our understanding of commonly circulating HCoVs and hopefully provide critical information about still-emerging coronaviruses.

2021-03-03 | GSE155986 | GEO

HCoV-229E infection of the human HuH7 cell line [Experiment A]

Project description:The aim of the experiment is to identify changes in host gene expression upon infection of HuH7 human hepatoma cells with Human corona virus-229E (HCoV-229E) compared to mock-infected cells. All experiments were performed at 33°C which is required for sufficient viral entry/replication.

2017-03-15 | GSE89160 | GEO

Cell host-response to infection with novel human coronavirus EMC predict potential antivirals and important differences with SARS-coronavirus.

Project description:Differential expression was determined in Calu-3 cells between mock infected and infection with either Human coronavirus EMC and SARS coronavirus at different times post infection. Calu-3 2B4 cells were infected with Human Coronavirus EMC 2012 (HCoV-EMC) or mock infected. Samples were collected 0, 3, 7, 12, 18 and 24 hpi. There are 3 mock and 3 infected replicates for each time point, except for 12 hpi for which there are only 2 infected replicates (one replicate did not pass RNA quality check). There were no mock sampes at 18 hpi, and therefore infected samples at 18 hpi were compared with mocks at 24 hpi. For direct comparison with SARS-CoV infected cells, raw data from HCoV-EMC experiments were quantile normalized together with the SARS-CoV dataset (GEO Series accession number GSE33267).

2013-05-03 | E-GEOD-45042 | biostudies-arrayexpress

Human Primary Airway Epithelium Response to HCoV-229E Infection

Project description:The purpose of this experiment was to evaluate the human host cellular response to wild-type Human coronavirus strain 229E (HCoV-229E) infection of primary human airway epithelial cell cultures. Sample data was obtained from mock-infected primary human airway epithelial cell cultures at 24 hours post infection. Primary human conducting airway epithelial cells were grown at air-liquid interface. Samples were harvested and processed for RNA sequencing (RNA-Seq) expression analysis and limited proteolysis proteomics. Wild-type human coronavirus strain 229E (HCoV-229E) infected and mock-infected primary human airway epithelial cell cultures with and without primary human lung macrophages were harvested at 24 hour post infection (MOI 3). Samples were processed for RNA sequencing (RNA-Seq; in Trizol) expression analysis and limited proteolysis proteomics (LiP). For LiP, cells were lysed by freeze thaw cycles and each replicate was split in half and treated for 1 minute with proteinase K and then trypsin and the other half just treated with trypsin prior to processing for proteomic analysis. Search for the DDA data was performed using MaxQuant, following the label-free quantification and match between runs (LFQ-MBR) workflow. The DIA data was searched with FragPipe/DIA-NN. The database search results were further processed using in-house codes which filtered and normalized the data, and added statistical parameters.

2025-11-12 | MSV000099878 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data