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Proteomic analysis (limited proteolysis and abundance based) of human coronavirus 229E infected immortalized human lung fibroblasts (MRC5) at 8, 16, and 24 hours post infection (whole cell lysate)

ABSTRACT: LiP assays performed on mock-infected and human coronavirus 229E-infected immortalized human lung fibroblasts (MRC5) cells (BTOL0001590). Samples were harvested at 8, 16, and 24 hours post infection (HCoV-299E). Samples are derived from human whole cell lysates (BTO:0004304). Mock-infected and human coronavirus 229E-infected immortalized human lung fibroblasts (MRC5) cells were collected at 8, 16, and 24 hours post infection (HCoV-299E). Cells were lysed by freeze thaw cycles and each replicate was split in half and treated for 1 minute with proteinase K and then trypsin and the other half just treated with trypsin prior to processing for proteomic analysis. The database searching was performed using FragPipe equipped with MSFragger, following the label-free quantification and match between runs (LFQ-MBR) workflow. The database search results were further processed using in-house codes which filtered and normalized the data, and added statistical parameters.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Human Coronavirus 229e (ncbitaxon:11137) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: John T. Melchior Amy Sims

PROVIDER: MSV000099901 | MassIVE | Thu Nov 13 22:15:00 GMT 2025

REPOSITORIES: MassIVE

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