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S. elongatus PCC 7942 Proteome Integral Solubility Alteration (PISA) Proteomics - Light vs Dark with Dense and Dilute

ABSTRACT: PISA experimental assays investigating structural alterations in Light vs Dark growth conditions with Dense and Dilute conditions. Samples are representative of Synechococcus elongatus PCC 7942 cell lysates (BTO:0004304). Purpose: Detecting structural and abundance changes in cyanobacterial cultures grown in conditions mimicking environmental perturbations such as dense light, dense dark, dilute light, and dilute dark. Technique: Proteome Integral Solubility Assay (PISA) and Global Proteomics. Other Details: TMT10 Labelled, Not-fractionated, 3 plexes in total (Plex1- PISA Dense Light and Dark, Plex2- PISA Dilute Light and Dark, Plex3- Global Dilute Light and Dark, No global proteomics for dense samples). Data was searched with MS-GF+ using PNNL's DMS Processing pipeline.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Synechococcus Elongatus Pcc 7942 (ncbitaxon:1140)

SUBMITTER: John T. Melchior Snigdha Sarkar

PROVIDER: MSV000099914 | MassIVE | Fri Nov 14 18:23:00 GMT 2025

SECONDARY ACCESSION(S): PXD070768

REPOSITORIES: MassIVE

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