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S. elongatus PCC 7942 Circadian Extracellular Sucrose Quantification Metabolomics

ABSTRACT: GC-MS extracellular sucrose quantification investigating metabolites involved in central carbon metabolism and photosynthetic electron transfer pathways in Synechococcus elongatus. Time-series laboratory adaptive evolution of Synechococcus elongatus PCC 7942 CscB/SPS strain (ncbitaxon:1140) under fluctuating oxygen stress conditions in controlled turbidostatic conditions. The starting strain (labelled WT) was grown between 0-78.4% oxygen before transferred to 0% oxygen. This process was repeated twice in adapted populated one (0-93%) and adapted population two (0-99.8%). A final sample was taken at 0% oxygen for adapted population 3.

INSTRUMENT(S): Agilent 7890A GC coupled to a 5975C inert XL MSD

ORGANISM(S): Synechococcus Elongatus Pcc 7942 (ncbitaxon:1140)

SUBMITTER: Pavlo Bohutskyi

PROVIDER: MSV000099915 | MassIVE | Fri Nov 14 18:51:00 GMT 2025

REPOSITORIES: MassIVE

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Project description:In the unicellular cyanobacterium, Synechococcus elongatus PCC 7942, essentially all promoter activities are under the control of the circadian clock in continuous light (LL) conditions. Here, we employed high-density oligonucleotide arrays to explore comprehensive profiles of genome-wide Synechococcus gene expression in wild type, kaiABC-null and kaiC-overexpressor strains under LL and continuous dark (DD) conditions. In the wild type strain more than 30% of transcripts significantly oscillated in a circadian fashion, peaking at subjective dawn and dusk. Such circadian control was nullified in kaiABC-null strains. Although KaiC has been proposed to globally repress gene expression, our analysis revealed that dawn expressing genes were upregulated by kaiC-overexpression, such that the clock was arrested at subjective dawn. Transfer of cells to continuous dark (DD) conditions from LL immediately suppressed expression of most of genes, while the clock keeps time even in the absence of transcriptional feedback. Thus, the Synechococcus genome seems primarily regulated by the light/dark cycles and dramatically modified by the protein-based circadian oscillator. Keywords: timecourse data (~48 hours under continuous light or darkness) from Synechococcus elongatus PCC 7942 (wild type, kaiABC-null, and inducible kaiC-overexpressor) strains Wild type (WT), kaiABC-null (Dkai), and inducible kaiC-overexpressor (oxC) S. elongatus PCC 7942 strains were analyzed under continuous light (LL), continuous dark (DD) using Affymetrix high-density oligonucleotide microarrays (GeneChip CustomExpress Arrays) representing predicted 2,515 protein-coding genes on the genome of Synechococcus elongatus PCC 6301, which can be used also to the almost homologous strain, S. elongatus PCC 7942: Two independent experiments in WT under LL (hours 0 to 52) and DD (hours 0 to 48); Two independent experiments in Dkai under LL (hours 0 to 48); Two independent experiments in oxC under LL in the presence or absence of an inducer, IPTG, from hour 25 to 33 in LL; a single experiment in WT under DD in the presence of an transcriptional inhibitor, rifampicin.

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S. elongatus PCC 7942 Circadian Extracellular Sucrose Quantification Metabolomics

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