The transmembrane protein disulfide isomerase TMX3 potentiates platelet aggregation and arterial thrombosis in mice

ABSTRACT: To identify the specific disulfide bonds in alphaIIbbeta3 catalyzed by TMX3, alphaIIbbeta3 protein was incubated with GSH (group 1), GSH plus TMX3 (group 2), or DTT (group 3). After incubation, free thiols were labeled with IAM, excess IAM was removed by Zeba column, and the remaining disulfide bonds were subsequently reduced by DTT and labeled with NEM, excess NEM was removed by Zeba column. The abundance of Cys-IAM was then quantified by mass spectrometry.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Fengwu Chen  

PROVIDER: MSV000099946 | MassIVE | Tue Nov 18 13:20:00 GMT 2025

SECONDARY ACCESSION(S): PXD070905

REPOSITORIES: MassIVE