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Label-free quantification of MCF10A cell culture proteome (DIA) stably expressing BAD3SA in 2D vs 3D

ABSTRACT: Global shotgun proteomic analysis using data independent acquisition (DIA) and Spectronaut analysis on the input cell lysates before the enrichment step in proximity labeling experiments, specifically MCF10A cells stably expressing Bcl2-associated agonist of cell death (BAD) with three serine to alanine mutations (S75A, S99A, S118A) fused with miniTurbo (miniTurbo-BAD3SA) in 2D with biotin treatment (n=3) and miniTurbo-BAD3SA in 3D with biotin treatment (n=4). The file "L20240424-miniTurbo-BAD-WT-vs-3SA-DIA.sne" contains Spectronaut search result including miniTurbo-BAD WT 2D and 3D shotgun samples found under the identifier MSV000094824 (2D) and MSV000094825 (3D).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Ing Swie Goping Olivier Julien

PROVIDER: MSV000100168 | MassIVE | Tue Dec 09 14:45:00 GMT 2025

SECONDARY ACCESSION(S): PXD071782

REPOSITORIES: MassIVE

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