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Proteomics of conditioned media used in the differentiation of equine umbilical cord derived mesenchymal stem cells

ABSTRACT: This proteomic study aimed to investigate the in vitro transdifferentiation potential of equine umbilical cord-derived mesenchymal stem cells (EqUC-MSCs) using a protocol based on conditioned medium derived from equine facial nerve explant culture. This study contributes to the development of novel cell-based strategies for peripheral nerve repair in equine medicine.

INSTRUMENT(S): Orbitrap Fusion Lumos Mass Spectrometer (Thermo Fisher Scientific)

ORGANISM(S): Equus Caballus (ncbitaxon:9796)

SUBMITTER: Rogerio Martins Amorim Lucas Vinicius de Oliveira Ferreira Lucilene Delazari dos Santos

PROVIDER: MSV000100207 | MassIVE | Fri Dec 12 16:09:00 GMT 2025

REPOSITORIES: MassIVE

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Project description:BACKGROUND: Perioperative neurocognitive disorder (PND) is a key complication affecting older individuals after anesthesia and surgery. Failure to translate multiple pharmacological therapies for PND from preclinical studies to clinical settings has necessitated the exploration of novel therapeutic strategies. Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) treatment has emerged as a promising therapeutic strategy for treating neurodegenerative diseases and has the potential to translate basic science into clinical practice. In this study, we investigated the effects and underlying mechanism of hUC-MSCs on PND in aged mice.METHODS: hUC-MSCs were isolated from an infant umbilical cord and identified using flow cytometry and differentiation assays. We established PND model by undergoing aseptic laparotomy under isoflurane anesthesia maintaining spontaneous ventilation in 18-month-old male C57BL/6 mice. hUC-MSCs were slowly injected into mice by coccygeal vein before anesthesia. Cognitive function, neuroinflammation, neuroplasticity, endogenous neurogenesis and brain-derived neurotrophic factor (BDNF) were assessed. To determine the mechanisms underlying by which hUC-MSCs mediate their neuroprotective effects in PND, K252a, an antagonist of BDNF receptor, was administered intraperitoneally before surgery. Hippocampal BDNF/TrkB/CREB signaling pathway and metabolomic signatures were evaluated.RESULTS: hUC-MSC treatment ameliorated the learning and memory impairment in aged mice with PND. The downstream effects were the suppression of neuroinflammatory responses and restoration of neurogenesis and neuroplasticity dysregulation. Interestingly, the level of mature BDNF, but not that of proBDNF, was increased in the hippocampus after hUC-MSC treatment. Further analysis revealed that the improved cognitive recovery and the restoration of neurogenesis and neuroplasticity dysregulation elicited by exposure to hUC-MSCs were, at least partially, mediated by the activation of the BDNF/TrkB/CREB signaling pathway. Untargeted metabolomic further identified lipid metabolism dysfunction as potential downstream of the BDNF/TrkB/CREB signaling pathway in hUC-MSC-mediated neuroprotection for PND.CONCLUSIONS: Our study highlights the beneficial effects of hUC-MSC treatment on PND and provides a justification to consider the potential use of hUC-MSCs in the perioperative period.

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Proteomics of conditioned media used in the differentiation of equine umbilical cord derived mesenchymal stem cells

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