Proteomics

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Waste water treatment plant effluent from Muenster, Germany


ABSTRACT: Samples were collected daily (19.06.2023-10.07.2023) the in the Waste water treatment plant "Rieselfelder" in Muenster, Germany, as full-day average sample. During the collection period, a festival took place in Muenster (24.06.2023). Field blanks of miliq were processed like the samples. 250 mL of sample were filtered 0.2 um cellulose acetate filters and extracted using Agilent Bond Elut PPL cartridges. Elution was performed with MeOH. Samples were reduced to dryness under an N2 stream and dissolved in 1 mL (MeOH:H2O (1:1, v/v)). UHPLC was performed using a Waters Acquity BEH C18 column (150 mm length, 1.7 um pore size). Eluents were miliq water (0.1% FA) and Acetonitrile (0.1% FA). Data was acquired on a timsTOF fleX in PASEF mode in positive and negative polarity and an Agilent 6530A qTOF in positive and negative polarity.

INSTRUMENT(S): 6530A Q-TOF LC/MS, timsTOF fleX

ORGANISM(S): Environmental Samples <bacillariophyta> (ncbitaxon:33858)

SUBMITTER: Steffen Heuckeroth  

PROVIDER: MSV000100240 | MassIVE | Tue Dec 16 06:04:00 GMT 2025

REPOSITORIES: MassIVE

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