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Proteomic analysis of phosphorylation in HCoV-229E-infected immortalized human lung fibroblasts (MRC5)

ABSTRACT: TMT18-labeled proteome-wide profiling of post-translational modifications (phosphorylation) in mock-infected and infected human lung epithelial cells (MRC5). Human lung epithelial (MRC5) cells were infected and mock-infected with HCoV-229E. Cells were harvested at 8, 16, and 24 hours post-infection. Extracted proteins were digested with trypsin, then TMT18-labeled and enriched for phosphorylation. Samples were run on the Q Exactive HF-X. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Human Coronavirus 229e (ncbitaxon:11137) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Amy Sims Tong Zhang

PROVIDER: MSV000100300 | MassIVE | Sun Dec 21 19:57:00 GMT 2025

SECONDARY ACCESSION(S): PXD072270

REPOSITORIES: MassIVE

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