Degradation of native and labelled linolenic acid by N. europaea

ABSTRACT: Nitrosomonas europaea Nm50 BNI degradation experiment:
- concentrate ~ 600 ml culture
- resuspend to make motherculture - 270 ml (need 255 for experiments)
- let acclimate back to 26C for ~20min- 1h without substrate
- add 1 mM NH4Cl (final concentration) to mother culture bottle:
- take T0 mother culture; take T1 mother culture (cca 20 min); T2 mother culture (40min);
split to treatments after 60 min

Treatments:
prepare 150 ul Linolenic acid (LNL) mix (50% unlabeled; 50% 13C LNL) on ice; after using
120 for inoculation of treatments; freeze the 30 ul mix @ -80
prepare just medium with 0.5 mM NH4

Biotic:
LNL; 8 ul of (50% unlabeled; 50% 13C LNL mix) - 5 replicates; 17 ml culture each (5)
PHA-LNL - 1 mM PHA+8 ul of (50% unlabeled; 50% 13C LNL mix) - 5 replicates; 17 ml
culture each (5)
control - nothing added - 5 replicates; 17 ml culture each (5)

Abiotic:
LNL - 8 ul of (50% unlabeled; 50% 13C LNL mix) - 5 replicates; 17 ml medium with 0.5 mM
NH4+ (5)

Sampling every 30-45 min; 5 timepoints

A: LE + culture
B: PHA-LE + culture
C: culture only
D: LE in media only

LC-HRMS according to 10.1007/s00216-025-05818-y

INSTRUMENT(S): Orbitrap IQ-X, Q Exactive HF

ORGANISM(S): Nitrosomonas Europaea (ncbitaxon:915)

SUBMITTER: christoph bueschl  

PROVIDER: MSV000100409 | MassIVE | Thu Jan 08 06:17:00 GMT 2026

REPOSITORIES: MassIVE