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Top-down Proteomics of Skinned Human Muscle Fibers Reveals Fiber-to-Fiber Variability

ABSTRACT: Method development for high sensitivity, surfactant-free intact protein extraction for single human muscle fibers (hSMFs) isolated from the vastus lateralis after membrane permeabilization and subsequent functional data collection. Includes qualitative assessment of variation in hSMFs from the same donor, and hSMFs from different donors.

INSTRUMENT(S): impact II, nanoACQUITY UPLC

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Ying Ge

PROVIDER: MSV000100493 | MassIVE | Thu Jan 15 11:23:00 GMT 2026

REPOSITORIES: MassIVE

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