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Cutibacterium acnes-induced T cell extracellular traps proteins

ABSTRACT: Cutibacterium acnes (C. acnes) is a common skin commensal associated with acne pathogenesis. Here, we investigated the capacity of acne-associated (CA) and healthy skin-associated (CH) C. acnes strains to induce extracellular trap (ET) formation in human neutrophils (NETs) and Th17 cells (TETs) and performed proteomic analyses to define their molecular signatures. NETs displayed a conserved protein profile regardless of the C. acnes strain, whereas TETs exhibited strain-specific proteomic profiles. CA-induced TETs were enriched in inflammatory and extracellular matrix remodeling proteins, while CH-induced TETs showed upregulation of nuclear, mitochondrial, and antimicrobial proteins, including granulysin, granzyme B, and cathepsin C. These findings underscore strain- and cell type-specific regulation of ET formation and suggest that CH-induced TETs may contribute to skin immune homeostasis, whereas CA-induced TETs may promote inflammatory and tissue remodeling responses in acne. Our study reveals a potential mechanism by which skin microbiota differentially modulates Th17 effector programs, ultimately influencing downstream immune activity and skin health.

INSTRUMENT(S): Q Exactive GC Orbitrap, nanoACQUITY UPLC

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: George Agak

PROVIDER: MSV000100784 | MassIVE | Mon Feb 09 16:25:00 GMT 2026

REPOSITORIES: MassIVE

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