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Photoaffinity labeling of human coronavirus 229E-infected MRC5 cells

ABSTRACT: Proteomic analysis of photoaffinity tagged methyltransferase inhibitor treated human fibroblasts infected with human coronavirus 229E. MRC5 cells were mock-infected (saline buffer treatment only) or infected with human coronavirus 229E in the presence or absence of the photoaffinity tagged methyltransferase inhibitor (10 and 40 micromolar) for 48 hours. Cells were UV treated, scraped, and lysed in 0.5% sodium dodecyl sulfate. Click chemistry was performed on samples normalized for protein concentration and samples were resolved on 10 to 20% Tris Glycine gels. Affinity probed targeted proteins were enriched using streptavidin beads prior to being submitted for proteomics. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Human Coronavirus 229e (ncbitaxon:11137) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Amy C. Sims Nicholas J. Reichart

PROVIDER: MSV000100843 | MassIVE | Fri Feb 13 11:31:00 GMT 2026

SECONDARY ACCESSION(S): PXD074443

REPOSITORIES: MassIVE

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