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Molecular mechanisms of growth differences in large yellow croaker: Metabolomic and metagenomic analyses

ABSTRACT:

Gut microbiota can modulate nutrient metabolism, considerably affecting host growth and development. However, mechanisms by which gut microbiota regulate large yellow croaker (LYC; Larimichthys crocea) growth remain unclear. We assessed gut contents of fast-growing male (IWHM) and female (IWHF) LYCs and slow-growing male (IWLM) and female (IWLF) LYCs and subjected the data to metagenomic and metabolomic analyses. Gut microbiota composition significantly differed among LYCs with different growth rates: Vibrio abundance was considerably lower in IWHM LYCs (2.53%) than in IWLM LYCs (41.47%); Arthrobacter D abundance was the highest in IWHF LYCs (8.19%) but the lowest in IWLF LYCs (1.52%). Moreover, 124 and 483 differential metabolites were noted in IWHM-IWLM and IWHF-IWLF LYC pairs, respectively. Finally, we established a model for gut microbiota-metabolite relationship in LYCs to understand molecular mechanisms by which gut microbiota regulates nutrient metabolism. Our results highlight differences in gut microbiota between LYCs with high and low growth rates and provide comprehensive insights into molecular mechanisms underlying LYC growth regulation by gut microbiota.

INSTRUMENT(S): Liquid Chromatography MS - positive - hilic, Liquid Chromatography MS - negative - hilic

PROVIDER: MTBLS10000 | MetaboLights | 2024-08-02

REPOSITORIES: MetaboLights

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Project description:Familial Mediterranean fever (FMF) is an inflammatory genetic disease characterized by elevated systemic reactivity against commensal gut microbiota and high levels of gut Candida albicans. The current study investigated the effects of Lactobacillus acidophillus INMIA 9602 Er 317/402 strain (probiotic “Narine”) on the relative abundance of gut enteric bacteria, lactobacilli, Staphylococcus aureus, and Enteroccocus faecalis in Candida albicans-carrier and non-carrier FMF patients in remission with the main MEFV mutation patterns M694V/V726A- the prevalent MEFV gene mutation within FMF patients in the Armenian cohort. Our data revealed that M694V/V726A mutations in PURIN inflammasome leading to FMF disease brought to gender specific differences in microbial community structure in FMF patients. Possibly, long-term colchicine use suppresses the PURIN inflammasome/inhibits NLRP3 inflammasome-dependent IL-1β release influencing on overgrowth of C. albicans in gut microbiota of FMF patients. The comparison of Operational Taxonomic Units (OTUs) of enteric bacteria in C. albicans-carrier and non-carrier female patients revealed the statistically significant increase in OTUs of enterobacteria in C. albicans-carriers. In contrast to this, there were no differences in abundance of Enteroccocus faecalis between female FMF C. albicans-carriers compared with non-carriers, while male FMF C. albicans-carriers have increased abundance of E. faecalis in their gut microbiota compared with that of male patients with none carriers. The gut microbiota of FMF patients (both male and female) with C. albicans below baseline level contains high abundance of lactobacilli compared with C. albicans-carriers. The adoption of Lactobacillus acidophilus INMIA 9602 Er 317/402 leads to changes in gut microbiota composition of FMF patients. It reduces, in particularly, the abundance of enterobacteria in females, and Enteroccocus faecalis in men parallel with reducing the numbers of yeast in gut microbiota of FMF patients. We hypothesize that colchicine treatment changes the already-altered gut microbiota of FMF patients, thereby affecting the regulation of immune system by inhibition of NLRP3 inflammasome. Colchicine could lead to overgrowth of C. albicans in gut microbiota of FMF patients, whereas the Lactobacillus acidophilus INMIA 9602 Er 317/402 works on activation of inflammasome by new changes in gut microbiota of patients.

			Action	DRS
	IWHF1_NEG.wiff	Wiff
	IWHF1_NEG.wiff.scan	Wiff
	IWHF2_NEG.wiff	Wiff
	IWHF2_NEG.wiff.scan	Wiff
	IWHF3_NEG.wiff	Wiff

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Molecular mechanisms of growth differences in large yellow croaker: Metabolomic and metagenomic analyses

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