Metabolomics

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A Pathogen-Responsive Gene Cluster for Highly Modified Fatty Acids in Tomato.


ABSTRACT: In response to biotic stress, plants produce suites of highly modified fatty acids that bear unusual chemical functionalities. Despite their chemical complexity and proposed roles in pathogen defense, little is known about the biosynthesis of decorated fatty acids in plants. Falcarindiol is a prototypical acetylenic lipid present in carrot, tomato, and celery that inhibits growth of fungi and human cancer cell lines. Using a combination of untargeted metabolomics and RNA sequencing, we discovered a biosynthetic gene cluster in tomato (Solanum lycopersicum) required for falcarindiol production. By reconstituting initial biosynthetic steps in a heterologous host and generating transgenic pathway mutants in tomato, we demonstrate a direct role of the cluster in falcarindiol biosynthesis and resistance to fungal and bacterial pathogens in tomato leaves. This work reveals a mechanism by which plants sculpt their lipid pool in response to pathogens and provides critical insight into the complex biochemistry of alkynyl lipid production.

INSTRUMENT(S): Liquid Chromatography MS - positive - reverse phase

SUBMITTER: Curt Fischer  

PROVIDER: MTBLS1039 | MetaboLights | 2022-01-17

REPOSITORIES: MetaboLights

Dataset's files

Source:
Action DRS
MTBLS1039 Other
13-124_12hpi_Chitin_1_150306.mzXML Mzxml
13-125_12hpi_Chitin_2_150306.mzXML Mzxml
13-126_12hpi_Chitin_3_150306.mzXML Mzxml
13-127_1dpi_Chitin_1_150306.mzXML Mzxml
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Publications

A Pathogen-Responsive Gene Cluster for Highly Modified Fatty Acids in Tomato.

Jeon Ju Eun JE   Kim Jung-Gun JG   Fischer Curt R CR   Mehta Niraj N   Dufour-Schroif Cosima C   Wemmer Kimberly K   Mudgett Mary Beth MB   Sattely Elizabeth E  

Cell 20200101 1


In response to biotic stress, plants produce suites of highly modified fatty acids that bear unusual chemical functionalities. Despite their chemical complexity and proposed roles in pathogen defense, little is known about the biosynthesis of decorated fatty acids in plants. Falcarindiol is a prototypical acetylenic lipid present in carrot, tomato, and celery that inhibits growth of fungi and human cancer cell lines. Using a combination of untargeted metabolomics and RNA sequencing, we discovere  ...[more]

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