Dataset Information
Multi-Omics Unveils Strain-Specific Neuroactive Metabolite Production Linked to Inflammation Modulation by Bacteroides and Their Vesicles
Ontology highlight
ABSTRACT: 18 Bacteroidetes isoaltes were analyzed for their neuroactive potentail using un-targeted and targeted metabolomics. The bacteria were grown to stationary phase, the supernatant was separated and purified using 0.22 um filter. The metabolome from the supernatant was extracted using standard Methanol extraction. The reconstituted supernatant samples were transferred into LC vials and loaded onto a sample tray in the nanoLC coupled to the Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific). Chromatographic separation of metabolites was performed on a Proxeon EASY nLC II System (Thermo Fisher Scientific) equipped with a Thermo Scientific Acclaim PepMap RSLC C18 column (P/N ES800A). The Xbridge BEH Amide (2.5 μm, 2.1 x 150 mm, Waters, Milford, MA, USA) column was used for metabolite separation.
INSTRUMENT(S): Liquid Chromatography MS - positive - reverse phase
PROVIDER: MTBLS10526 | MetaboLights | 2025-08-12
REPOSITORIES: MetaboLights
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| B3S.mzML | Mzml | |||
| BH19.mzML | Mzml | |||
| BH24_72.mzML | Mzml | |||
| BH27b_72.mzML | Mzml | |||
| BH30_72.mzML | Mzml |
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Publications
Multi-omics unveils strain-specific neuroactive metabolite production linked to inflammation modulation by <i>Bacteroides</i> and their extracellular vesicles.
Current research in microbial sciences 20250205
<i>Bacteroides</i> species are key members of the human gut microbiome and play crucial roles in gut ecology, metabolism, and host-microbe interactions. This study investigated the strain-specific production of neuroactive metabolites by 18 Bacteroidetes (12 <i>Bacteroides</i>, 4 <i>Phocaeicola</i>, and 2 <i>Parabacteroides</i>) using multi-omics approaches. Genomic analysis revealed a significant potential for producing GABA, tryptophan, tyrosine, and histidine metabolism-linked neuroactive com ...[more]
