Dataset Information

Effects of gut microbiota on the susceptibility of ischemic stroke in mice

ABSTRACT:

Ischemic stroke is a highly prevalent disease with constrained therapeutic options, and recent studies suggest that gut microbiota significantly influences its development. However, it remains unclear whether the gut microbiota has an impact on the susceptibility of ischemic stroke. Here, we aimed to investigate the role of the gut microbiota in the susceptibility of ischemic stroke.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS10583 | MetaboLights | 2026-02-11

REPOSITORIES: MetaboLights

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	a_MTBLS10583_LC-MS_negative_reverse-phase_metabolite_profiling.txt	Txt
	a_MTBLS10583_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS10583_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular
	m_MTBLS10583_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular

Items per page:

1 - 5 of 6

Similar Datasets

Project description:Abstract: Background and Objective. As a prevalent cerebrovascular disorder, the association between ischemic stroke and gut microbiota has garnered increasing attention. Recent studies have confirmed that astaxanthin exhibits significant protective effects against ischemic stroke. However, there is still a lack of clear experimental evidence regarding whether astaxanthin can regulate the gut microbiome in patients with ischemic stroke.Methods. To investigate the neuroprotective mechanism of astaxanthin and its regulatory effect on gut microbiota, a rat stroke model was established using the middle cerebral artery occlusion method. Molecular docking and molecular dynamics were used to verify the binding stability of energy metabolism-related proteins with astaxanthin. The intervention effects of astaxanthin on ischemic brain injury were systematically evaluated through histopathological analysis (hematoxylin-eosin staining) and molecular biology assays (ELISA and Western blot). Additionally, the composition of gut microbiota was analyzed using 16S rRNA high-throughput sequencing technology, combined with untargeted metabolomics methods, to comprehensively elucidate the regulatory effects of astaxanthin on brain metabolites, thereby clarifying its potential molecular mechanisms. Results. Astaxanthin intervention significantly altered the structure of the gut microbiota, characterized by an increase in beneficial bacteria and a decrease in pathogenic bacteria, which concurrently led to marked changes in brain metabolites. The results of molecular docking and molecular dynamics show that astaxanthin has stable binding to the energy metabolism proteins AMPK and SIRT1. This protective mechanism not only improved the energy metabolism levels under ischemic stroke conditions but also involved the upregulation of AMPK and SIRT1 expression.Conclusions. Research indicates that astaxanthin may exert neuroprotective effects against ischemic stroke through the AMPK/SIRT1 signaling pathway, with its mechanism involving the regulation of gut microbiota and their metabolic products. Based on this finding, this compound holds significant research value as a potential therapeutic agent, warranting further exploration and validation.

Dataset Information

Effects of gut microbiota on the susceptibility of ischemic stroke in mice

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets