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Integrated metabolomics and transcriptomics analysis elucidate the candidate genes for polyphyllin biosynthesis in Paris

ABSTRACT:

Polyphyllins are the key components in Paris, with varying levels and compositions among different species. This study investigated polyphyllin compositions of 206 Paris samples from 16 different producing areas. 9 Paris polyphylla var. yunnanensis, 3 Paris fargesii and 3 Paris forrestii were selected based on polyphyllin levels and composition for metabolome and transcriptome analysis. A total of 1243 metabolites and 1007 SCMs were identified among these samples, along with 92.11 Gb of full-length transcriptome data. Through a comprehensive analysis of the metabolome and transcriptome, a total of six 2,3-oxidized squalene cyclases (OSCs), 120 cytochrome P450s (CYPs) and 138 UDP-glycosyltransferases (UGTs) were annotated. Phylogenetic tree analysis narrowed down the candidate genes for OSC, CYP and UGT to 6, 12 and 26, respectively. By integrating metabolic and genetic differences, a total of 17 candidate genes including 2 OSCs, 4 CYPs and 11 UGTs, were identified. This study provides potential candidate genes involved in polyphyllins synthesis in Paris, aiming to facilitate the biosynthesis of different polyphyllins.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS10682 | MetaboLights | 2024-10-18

REPOSITORIES: MetaboLights

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			Action	DRS
	LC-1_N.mzML	Mzml
	LC-1_P.mzML	Mzml
	LC-2_N.mzML	Mzml
	LC-2_P.mzML	Mzml
	LC-3_N.mzML	Mzml

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