Polymicrobial detection and metabolomics in saliva of children with early childhood caries
Ontology highlight
ABSTRACT: Cariogenic microorganisms as well as salivary metabolic status in early childhood caries (ECC) have been reported to differ from that in adult caries. The main aim of this study was to identify specific microbial species and salivary metabolites in children with ECC using species-specific qPCR and untargeted metabolomic approach. Scardovia wiggsiae, Streptococcus mutans, Streptococcus sobrinus, Lactobacillus salivarius and Candida albicans were more prevalent in ECC group. Prevalence of ECC was higher in children with two targeted species present. Histidine metabolism and valine, leucine and isoleucine degradation were activated in ECC group, while glyoxylate and dicarboxylate metabolism, Purine and Pyrimidine metabolism were inhibited. Histidine and glutathione metabolism was activated with enrichment of targeted microorganism, while linoleic acid and biotin metabolism was inhibited. These findings suggest that detection / co-detection of targeted microorganisms can well describe caries status in children. Microbial and metabolite biomarkers can be used in caries control for children.
INSTRUMENT(S): Liquid Chromatography MS - alternating - reverse phase
PROVIDER: MTBLS10890 | MetaboLights | 2024-11-12
REPOSITORIES: MetaboLights
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Project description:<p>Metabolites are important determinants of disease progression. In this study, we conducted a case-control study to explore permanent teeth caries-related differences in salivary metabolite profiles and related changes in the composition of the oral microbiota in 200 subjects including 100 caries-free individuals and 100 with moderate-to-severe caries with good homogeneity through widely targeted metabolomics and 16S rRNA deep sequencing technologies. We found that: 1) 822 metabolites were detectable in salivary samples from these participants, of which 4-acetamidobutyric acid and adipic acid were respectively enriched in healthy individuals and individuals with caries, exhibited the greatest area under the curve (AUC) values of > 0.970; 2) less diversity was observed in individuals with dental caries relative to caries-free individuals, and significant differences in the dominant taxa were evident between the two groups. For example, the healthy oral microbiome is enriched for <em>Rothia aeria</em> and <em>Neisseria perflava</em>, whereas <em>Prevotella melaninogenica</em>, <em>Streptococcus salivarius</em>, <em>Streptococcus parasanguinis</em> and <em>Prevotella histicola</em>, and <em>Streptococcus mutans</em> were enriched in individuals with caries; 3) <em>Prevotella histicola</em>, <em>Oribacterium asaccharolyticum </em>and<em> Rothia aeria</em> were the top 3 species being required to differentiate caries from caries free population; 4) adipic acid and 4-acetamidobutyric acid derived from microbiota and host-microbiota co-metabolism, respectively. A negative correlation was observed between the microbes enriched in caries individuals (such as <em>Prevotella histicola</em>) and 4-acetamidobutyric acid. Moreover, the upstream regulatory factor ALDH of 4-acetamidobutyric acid is expressed throughout the entire process of tooth development including the differentiation of tooth bud, ameloblasts and odontoblasts. Together, these results suggest that 4-acetamidobutyric acid and adipic acid are important targets for future caries-focused research, offering insight into cariogenic activity or risk assessment in moderate or severe cases.</p>
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